Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62595
Jacquemin P, et al., Transcription factor hepatocyte nuclear factor 6 regulates pancreatic endocrine cell differentiation and controls expression of the proendocrine gene ngn3. Mol Cell Biol. 2000 Jun;20(12):4445-54
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7718662
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-Isl-1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4K:
embryonic day 10.75
(more  )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
4K:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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Specimen
4L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
4M:
embryonic day 12.5
(more )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
4M:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
Clusters |
4M
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Expression in clusters of cells.
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Specimen
4N:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Weak |
Clusters |
4N
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Markedly reduced numbers of expressing cells compared to control. Expression in at normal levels within clusters of cells.
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Specimen
4O:
embryonic day 15.5
(more )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
4O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Clusters |
4O
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Expression in clusters of cells.
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Specimen
4P:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Weak |
Clusters |
4P
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Markedly reduced numbers of expressing cells compared to control. Expression in at normal levels within clusters of cells.
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| TS23: pancreas epithelium |
Present |
Regionally restricted |
4P
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Expression in a few cells in epithelium lining cysts.
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Analysis Tools