Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62595
Jacquemin P, et al., Transcription factor hepatocyte nuclear factor 6 regulates pancreatic endocrine cell differentiation and controls expression of the proendocrine gene ngn3. Mol Cell Biol. 2000 Jun;20(12):4445-54
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7718629
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Gene symbol:
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Gcg
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Gene name:
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glucagon
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Antibody:
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anti-GLU 001
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2A:
embryonic day 10.75
(more  )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: pancreas primordium |
Present |
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2B
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Normal number of expressing cells compared to control.
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Specimen
2C:
embryonic day 12.5
(more )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Present |
Clusters |
2D
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Number of expressing cells was reduced by 85% compared to control, but number of clusters of expressing cells was normal.
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| TS20: pancreas epithelium |
Present |
Regionally restricted |
2D
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Expressing cells interspersed in epithelium.
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Specimen
2E:
embryonic day 15.5
(more )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Clusters |
2F
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Number of expressing cells was reduced by 85% compared to control, but number of clusters of expressing cells was normal.
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| TS23: pancreas epithelium |
Present |
Regionally restricted |
2F
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Expressing cells interspersed in epithelium.
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Specimen
2G:
postnatal day 4
(more )
Note: Genetic background of control was either wild type or heterozygous.
Specimen
2G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Genetic background of control was either wild type or heterozygous.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
pancreatic A cell |
Present |
Clusters |
2G
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Expression in alpha cells clustered in islets.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
pancreatic A cell |
Present |
Scattered |
2H
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Markedly reduced number of alpha cells compared to control. Expressing cells scattered within and in vicinity of pancreatic ducts.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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postnatal week 5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreatic duct |
Present |
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3J
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Expression in cells within or in close association with pancreatic ducts.
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Analysis Tools