Gene Expression Data
Assay Details
Assay
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Reference:
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J:315095
Bando Y, et al., Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice. J Mol Histol. 2014 Oct;45(5):507-18
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7716435
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Gene symbol:
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Fabp5
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Gene name:
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fatty acid binding protein 5, epidermal
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Antibody:
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Anti-EFABP
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2a:
postnatal week 4
(more )
Note: Double labeled: red - Fabp5; green - Lgals3 (assay MGI:7716436).
Specimen
2a:
(close )
Genetic Background:
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DDY
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Fabp5; green - Lgals3 (assay MGI:7716436).
|
Specimen
8a:
postnatal week 4
(more )
Note: Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
Specimen
8a:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal week 4
|
Sex:
|
Male
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: epiphyseal plate ossification zone |
perivascular cell |
Present |
|
8a
|
Septoclasts at postnatal week 4 had several long processes that elongated
toward the transverse septa.
|
Specimen
8b:
postnatal week 4
(more )
Note: Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
Specimen
8b:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal week 4
|
Sex:
|
Male
|
Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: epiphyseal plate ossification zone |
perivascular cell |
Present |
|
8b
|
A number of short (approximately 3 um in length) spines from the septoclast apexes were observed.
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Specimen
8c:
postnatal week 9
(more )
Note: Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
Specimen
8c:
(close )
Genetic Background:
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DDY
|
Age:
|
postnatal week 9
|
Sex:
|
Male
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: epiphyseal plate ossification zone |
perivascular cell |
Present |
|
8c
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In postnatal week 9 mice, immunopositive septoclasts were smaller than those of postnatal week 4 mice and there were fewer cell processes.
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Specimen
8d:
postnatal week 9
(more )
Note: Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
Specimen
8d:
(close )
Genetic Background:
|
DDY
|
Age:
|
postnatal week 9
|
Sex:
|
Male
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Confocal laser-scanning of 20 um thick sections was used for three-dimensional analysis.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: epiphyseal plate ossification zone |
perivascular cell |
Present |
|
8d
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Short (approximately 1 um) spines extended from the septoclast apexes to the transverse septa.
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