Gene Expression Data
Assay Details
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Assay
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Reference:
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J:123041
Izzi L, et al., Foxh1 recruits Gsc to negatively regulate Mixl1 expression during early mouse development. EMBO J. 2007 Jul 11;26(13):3132-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7715160
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Gene symbol:
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Mixl1
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Gene name:
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Mix paired-like homeobox
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Probe:
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Mixl1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B E7.0 Foxh1+/+:
embryonic day 7.0
(more  )
Specimen
2B E7.0 Foxh1+/+:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B E7.0 Foxh1-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Strong |
Regionally restricted |
2B E7.0 Foxh1-/-
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Expression within posterior primitive streak upregulated compared to wild type.
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| TS10: embryo mesoderm |
Strong |
Regionally restricted |
2B E7.0 Foxh1-/-
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Expression within wings of nascent mesoderm upregulated compared to wild type.
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Specimen
2B E7.5 Foxh1+/+:
embryonic day 7.5
(more )
Specimen
2B E7.5 Foxh1+/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
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2B E7.5 Foxh1+/+
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n limited to primitive streak.
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Specimen
2B E7.5 Foxh1-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Strong |
Regionally restricted |
2B E7.5 Foxh1-/-
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Expression within posterior primitive streak upregulated compared to wild type.
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
2B E7.5 Foxh1-/-
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Expression expanded anteriorly into mesoderm.
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| TS11: conceptus |
Present |
Regionally restricted |
2B E7.5 Foxh1-/-
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Expression expanded proximally in embryonic-extraembryonic border.
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Specimen
2B E8.0 Foxh1+/+:
embryonic day 8.0
(more )
Specimen
2B E8.0 Foxh1+/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
Regionally restricted |
2B E8.0 Foxh1+/+
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Expression restricted to posterior primitive streak.
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| TS12: allantois |
Present |
Regionally restricted |
2B E8.0 Foxh1+/+
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Expression at base of allantois.
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Specimen
2B E8.0 Foxh1-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxh1tm1Jlw/Foxh1tm1Jlw
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
Regionally restricted |
2B E8.0 Foxh1-/-
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Expanded expression domain in caudal end of primitive streak.
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| TS12: embryo endoderm |
Present |
Regionally restricted |
2B E8.0 Foxh1-/-
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Ectopic domain of expression in anterior endoderm overlying head process.
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Analysis Tools