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Gene Expression Data
Assay Details
Assay
Reference: J:74110 Ohno M, et al., Allele-specific detection of nascent transcripts by fluorescence in situ hybridization reveals temporal and culture-induced changes in Igf2 imprinting during pre-implantation mouse development. Genes Cells. 2001 Mar;6(3):249-59
Assay type: RNA in situ
MGI Accession ID: MGI:7715151
Gene symbol: Igf2
Gene name: insulin-like growth factor 2
Probe: Igf2 probe
Probe preparation: labelled with digoxigenin DNA
Visualized with: Fluorescein
Results
Specimen not shown: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent Expression undetectable in nuclei of one-cell embryos.
Specimen 3A: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 3A Transcription first detected at two-cell stage.
Specimen 3B: embryonic day 2.5 (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 3B Biallelic transcription predominant in nuclei of 8-cell embryos.
Specimen 3C: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 3C Increased percentage of nuclei showing monoallelic transcription. Some nuclei still show biallelic transcription.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory