Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43434
George EL, et al., Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood. 1997 Oct 15;90(8):3073-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7715143
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3A:
embryonic day 8.0
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: endocardial tube |
Present |
Regionally restricted |
3A
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Expression in endocardial tube displaying a prominent lumen.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: endocardial tube |
Present |
Regionally restricted |
3B
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Expression in collapsed endocardial tube.
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: endocardial tube |
Present |
Regionally restricted |
3C
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Expression in a thin, single cell thick, line of cells between early myocardial cells and endoderm.
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Specimen
3D:
embryonic day 8.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: endocardial tube |
Present |
Regionally restricted |
3D
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Expression in looped endocardial tube with lumen.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: endocardial tube |
Present |
Regionally restricted |
3E
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Prominent expression in endocardial tube lacking lumen.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: endocardial tube |
Present |
Regionally restricted |
3F
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Prominent expression in endocardial tube lacking lumen.
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Specimen
5A:
embryonic day 8.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac blood vessel |
blood vessel endothelial cell |
Present |
Regionally restricted |
5A
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Expression in endothelial cells lining newly formed blood vessels.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac mesenchyme |
Present |
Regionally restricted |
5B
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Expression in yolk sac mesoderm, not distributed as a lining for blood vessels.
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Specimen
5C:
embryonic day 8.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS13: dorsal aorta |
aortic endothelial cell |
Present |
Regionally restricted |
5C
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Expression in endothelial cells lining dorsal aorta in tight contact with surrounding mesoderm.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Fn1tm1Hyn/Fn1tm1Hyn
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS13: dorsal aorta |
aortic endothelial cell |
Present |
Regionally restricted |
5D
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Expression in endothelial cells separated from surrounding mesoderm.
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