Gene Expression Data
Assay Details
Assay
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Reference:
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J:154889
Deschamps C, et al., Expression of ephrinA5 during development and potential involvement in the guidance of the mesostriatal pathway. Exp Neurol. 2009 Oct;219(2):466-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7714980
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Gene symbol:
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Slc6a3
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Gene name:
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solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
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Antibody:
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anti-DAT
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
8b/c:
embryonic day 16.5
(more )
Note: Double labeled: red - Slc6a3; green - Efna5 (assay MGI:7714979).
Specimen
8b/c:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc6a3; green - Efna5 (assay MGI:7714979).
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Structure |
Level |
Pattern |
Image |
Note |
TS24: thalamus |
Present |
Regionally restricted |
8c
,
8b
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Expression was distributed ventrally to the Efna5+ region.
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Specimen
8e/f:
embryonic day 18.5
(more )
Note: Double labeled: red - Slc6a3; green - Efna5 (assay MGI:7714979).
Specimen
8e/f:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc6a3; green - Efna5 (assay MGI:7714979).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: corpus striatum |
Present |
Regionally restricted |
8f
,
8e
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Positive mesencephalic dopaminergic axons grew towards the dorsal part of the striatum where low Efna5 expression was detected, whereas no positive axons
crossed the most ventral and rostral part of the striatum expressing high Efna5 levels.
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