Probe:	Spatial-epsilon cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1A E10.5

Structure	Level	Pattern	Image	Note
TS19: 3rd branchial pouch	Present		1A E11.5

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Present		1A E12.5	A large distribution of expression was seen in the thymic rudiment.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present	Widespread	1A E15.5	Expression persisted throughout the thymus.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: thymus	epithelial cell of thymus	Present	Regionally restricted	1A E17.5	Expression was detected in an abundant subset of thymic epithelial cells in the subcapsular region.
TS25: thymus		Present	Scattered	1A E17.5	A more subtle expression was in scattered cells of other compartments of the thymus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus subcapsular epithelium	epithelial cell of thymus	Present		1D C57BL/6J top	Expressing thymic epithelial cells are mainly concentrated in the subcapsular region of the adult thymus.
TS28: thymus cortex epithelium	cortical thymic epithelial cell	Present		1D C57BL/6J top	Expression in the cortex is diluted due to the presence of thymocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus epithelium	epithelial cell of thymus	Present		1D CD3e-/- top	Expression showed a differential distribution in the mutant.

Structure	Level	Pattern	Image	Note
TS19: 3rd branchial pouch	Present		S1_bottom	Expression was in the thymic and the parathyroid domains.