Gjb4 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:140005 Zheng-Fischhofer Q, et al., Characterization of connexin30.3-deficient mice suggests a possible role of connexin30.3 in olfaction. Eur J Cell Biol. 2007 Dec;86(11-12):683-700
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7714708
Gene symbol:	Gjb4
Gene name:	gap junction protein, beta 4

Reporter:	lacZ
Antibody:	anti-B-galactosidase (Abcam)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

Specimen 3C: postnatal adult; Gjb4^tm1Kwi/Gjb4^tm1Kwi (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical collecting duct	Present	Single cells	3C	A few cells of the cortex were labeled.
TS28: renal proximal tubule	Present	Single cells	3C	A few cells of the cortex were labeled.

Specimen 3D: postnatal adult; Gjb4^tm1Kwi/Gjb4^tm1Kwi (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thin ascending limb of inner medulla		Present	Regionally restricted	3D	The most intense labelling was found in the inner medulla in the loop of Henle.
TS28: glomerular tuft		Absent		3D
TS28: medullary collecting duct		Absent		3D
TS28: renal vasculature	erythrocyte	Absent		3D

Specimen 3E: postnatal adult; Gjb4^tm1Kwi/Gjb4^tm1Kwi (more

)
Note: Double labeled: red - Gjb4 reporter; green - Aqp1 (assay MGI:7714709).

Structure	Cell Type	Level	Image	Note
TS28: thin ascending limb of inner medulla		Present	3E	Beta-galactosidase nuclear staining was localized in thin-walled tubular structures devoid of Aquaporin 1.
TS28: medullary collecting duct		Absent	3E
TS28: renal vasculature	erythrocyte	Absent	3E

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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