Gene Expression Data
Assay Details
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Assay
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Reference:
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J:329933
Akkuratova N, et al., Developmental heterogeneity of embryonic neuroendocrine chromaffin cells and their maturation dynamics. Front Endocrinol (Lausanne). 2022;13:1020000
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7713493
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Gene symbol:
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Myl1
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Gene name:
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myosin, light polypeptide 1
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Probe:
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Mm-Myl1 (548421)
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Probe preparation:
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Antisense DNA
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Visualized with:
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Fluorescence
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Results
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Specimen
7A E14.5:
embryonic day 14.5
(more  )
Note: Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
Specimen
7A E14.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: adrenal medulla |
adrenal medulla chromaffin cell |
Present |
Regionally restricted |
7A E14.5 Inset lower left
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7A E14.5
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7A E14.5 Inset top left
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Expression was detected in roughly 50% of all chromaffin cells (identified by Th immunostaining).
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| TS22: adrenal medulla |
type I cell of adrenal medulla |
Present |
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7A E14.5 Inset top left
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7A E14.5
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7A E14.5 Inset lower left
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Expression was detected in noradrenergic chromaffin cells.
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Specimen
7A E16.5:
embryonic day 16.5
(more )
Note: Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
Specimen
7A E16.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: adrenal medulla |
adrenal medulla chromaffin cell |
Present |
Regionally restricted |
7A E16.5 Inset lower left
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7A E16.5 Inset top left
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7A E16.5
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Expression was detected in roughly 50% of all chromaffin cells (identified by Th immunostaining).
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| TS24: adrenal medulla |
type I cell of adrenal medulla |
Present |
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7A E16.5 Inset top left
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7A E16.5
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7A E16.5 Inset lower left
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Expression was detected in noradrenergic chromaffin cells.
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Specimen
7A E18.5:
embryonic day 18.5
(more )
Note: Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
Specimen
7A E18.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: adrenal medulla |
adrenal medulla chromaffin cell |
Present |
Regionally restricted |
7A E18.5
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7A E18.5 Inset lower left
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7A E18.5 Inset top left
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Expression was detected in roughly 50% of all chromaffin cells (identified by Th immunostaining).
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| TS26: adrenal medulla |
type I cell of adrenal medulla |
Present |
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7A E18.5 Inset lower left
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7A E18.5 Inset top left
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7A E18.5
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Expression was detected in noradrenergic chromaffin cells.
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Specimen
7A P2:
postnatal day 2
(more )
Note: Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
Specimen
7A P2:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Myl1; red - Rgs5 (assay MGI:7713486); blue - Th (assay MGI:7713488).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: adrenal medulla |
adrenal medulla chromaffin cell |
Present |
Regionally restricted |
7A P2
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7A P2 Inset top left
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7A P2 Inset lower left
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Expression was downregulated postnatally to 5.99% of all chromaffin cells (identified by Th immunostaining) at P2.
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| TS27: adrenal medulla |
type I cell of adrenal medulla |
Present |
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7A P2 Inset top left
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7A P2 Inset lower left
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7A P2
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Expression was detected in noradrenergic chromaffin cells.
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Analysis Tools