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Gene Expression Data
Assay Details
Assay
Reference: J:293992 Mancia Leon WR, et al., Clustered gamma-protocadherins regulate cortical interneuron programmed cell death. Elife. 2020 Jul 7;9:e55374
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7713333
Gene symbol: Reln
Gene name: reelin
Antibody: anti-CR-50
Results
Specimen 2-S1A Gad2Cre;Ai14;WT: postnatal day 30; Gad2tm2(cre)Zjh/Gad2+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 2-S1A Gad2cre;Ai14;WT RNL A subset of cortical inhibitory neurons (derived from both the medial and caudal ganglionic eminences) were stained.
Specimen 2-S1A Gad2Cre;Ai14;Pcdhgfcon3/+: postnatal day 30; Gad2tm2(cre)Zjh/Gad2+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhg+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 2-S1A Gad2cre;Ai14;Pcdhgfcon3/+ RNL A subset of cortical inhibitory neurons (derived from both the medial and caudal ganglionic eminences) were stained.
Specimen 2-S1A Gad2Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 30; Gad2tm2(cre)Zjh/Gad2+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 2-S1A Gad2cre;Ai14;Pcdhgfcon3/fcon3 RNL The density of cortical inhibitory neurons stained positive was significantly reduced in the visual cortex of homozygous Gad2<Cre>;Ai14;Pcdhg<fcon3/fcon3>.
Specimen 3-S1A Nkx2.1Cre;Ai14;WT: postnatal day 30; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 3-S1A Nkx2.1cre;Ai14;WT RNL A subset of cortical inhibitory neurons (derived from both the medial and caudal ganglionic eminences) were stained.
Specimen 3-S1A Nkx2.1Cre;Ai14;Pcdhgfcon3/+: postnatal day 30; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhg+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 3-S1A Nkx2.1cre;Ai14;Pcdhgfcon3/+ RNL A subset of cortical inhibitory neurons (derived from both the medial and caudal ganglionic eminences) were stained.
Specimen 3-S1A Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 30; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 3-S1A Nkx2.1cre;Ai14;Pcdhgfcon3/fcon3 RNL A smaller, but significant reduction in the immunopositive cortical inhibitory neuron population was observed, in agreement with the notion that a subpopulation of Reln+ cells is born in the medial ganglionic eminence.
Specimen 3-S2A Nkx2.1Cre;Ai14: postnatal day 30; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: green - Reln; red - tdTomato.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 3-S2A Nkx2.1cre;Ai14 right , 3-S2A Nkx2.1cre;Ai14 left A subset of cortical inhibitory neurons (derived from both the medial and caudal ganglionic eminences) were stained.
Specimen 3-S2A Nkx2.1Cre;Ai14;Pcdhgfcon3/fcon3: postnatal day 30; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pcdhgtm2Xzw/Pcdhgtm2Xzw, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: green - Reln; red - tdTomato.
Structure Cell Type Level Pattern Image Note
TS28: primary visual cortex inhibitory interneuron Present Regionally restricted 3-S2A Nkx2.1cre;Ai14;Pcdhgfcon3/fcon3 left , 3-S2A Nkx2.1cre;Ai14;Pcdhgfcon3/fcon3 right Layer 1 Reln+ cells, which are largely derived from the caudal ganglionic eminence were not affected in the mutant. Immunopositive cells in deeper layers 2-6 (many of which are medial ganglionic eminence-derived) showed reduced numbers in the visual cortex of homozygous Nkx2.1<Cre>;Ai14;Pcdhg<fcon3/fcon3>.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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