Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74066
Fauchey V, et al., Dopamine control of striatal gene expression during development: relevance to knockout mice for the dopamine transporter. Eur J Neurosci. 2000 Sep;12(9):3415-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7713127
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Gene symbol:
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Penk
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Gene name:
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preproenkephalin
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Probe:
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PPA probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
PPA G14 not shown:
embryonic day 14.0
(more  )
Specimen
PPA G14 not shown:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
|
embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
Non-Uniform |
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Heterogeneous expression in striatal anlage from G14.
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Specimen
PPA G16 not shown:
embryonic day 16.0
(more )
Specimen
PPA G16 not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
|
embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Present |
Graded |
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Expression heterogeneously distributed throughout striatum, with increasing dorsoventral gradient of expression
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Specimen
2C PPA DAT+/+:
embryonic day 17.0
(more )
Specimen
2C PPA DAT+/+:
(close )
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Genetic Background:
|
involves: 129P2/OlaHsd
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Age:
|
embryonic day 17.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Present |
Graded |
2C PPA DAT+/+
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Expression heterogeneously distributed throughout striatum, with increasing dorsoventral gradient of expression
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Specimen
2C PPA DAT-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Weak |
Non-Uniform |
2C PPA DAT-/-
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Expression heterogeneously distributed. Expression decreased compared to wild type.
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Specimen
3C PPA DAT+/+:
postnatal day 2
(more )
Specimen
3C PPA DAT+/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Graded |
3C PPA DAT+/+
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Expression heterogeneously distributed throughout striatum, with increasing dorsoventral gradient of expression
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Specimen
3C PPA DAT-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Weak |
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3C PPA DAT-/-
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Expression decreased compared to wild type.
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Specimen
PPA whole striatum G13 +/+:
embryonic day 13.0
(more )
Specimen
PPA whole striatum G13 +/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA whole striatum G13 -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA whole striatum G14 +/+:
embryonic day 14.0
(more )
Specimen
PPA whole striatum G14 +/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
|
embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA whole striatum G14 -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA whole striatum G16 +/+:
embryonic day 16.0
(more )
Specimen
PPA whole striatum G16 +/+:
(close )
|
Genetic Background:
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involves: 129P2/OlaHsd
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Age:
|
embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA whole striatum G16 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
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|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
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Not Specified
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Type:
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section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Weak |
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|
Expression first detected in mutant at G16. Expression decreased 47% compared to wild type.
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Specimen
PPA whole striatum G17 +/+:
embryonic day 17.0
(more )
Specimen
PPA whole striatum G17 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 17.0
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|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA whole striatum G17 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
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|
Age:
|
embryonic day 17.0
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|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex |
Weak |
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Expression decreased by 26% compared to wild type.
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Specimen
PPA caudate-putamen P0 +/+:
postnatal day 0
(more )
Specimen
PPA caudate-putamen P0 +/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA caudate-putamen P0 -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: caudate-putamen |
Weak |
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Expression decreased by 38% compared to wild type.
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Specimen
PPA caudate-putamen P2 +/+:
postnatal day 2
(more )
Specimen
PPA caudate-putamen P2 +/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA caudate-putamen P2 -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: caudate-putamen |
Weak |
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|
Expression decreased by 28% compared to wild type.
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Specimen
PPA caudate-putamen P7 +/+:
postnatal day 7
(more )
Specimen
PPA caudate-putamen P7 +/+:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
PPA caudate-putamen P7 -/-:
(close )
|
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: caudate-putamen |
Weak |
|
|
Expression decreased by 31% compared to wild type.
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Specimen
PPA caudate-putamen Adult +/+:
postnatal adult
(more )
Specimen
PPA caudate-putamen Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA caudate-putamen Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: caudate-putamen |
Weak |
|
|
Expression decreased by 26% compared to wild type.
|
Specimen
PPA nucleus accumbens P0 +/+:
postnatal day 0
(more )
Specimen
PPA nucleus accumbens P0 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
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Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens P0 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens P2 +/+:
postnatal day 2
(more )
Specimen
PPA nucleus accumbens P2 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens P2 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens P7 +/+:
postnatal day 7
(more )
Specimen
PPA nucleus accumbens P7 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens P7 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens Adult +/+:
postnatal adult
(more )
Specimen
PPA nucleus accumbens Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
PPA nucleus accumbens Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools