Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:74066
Fauchey V, et al., Dopamine control of striatal gene expression during development: relevance to knockout mice for the dopamine transporter. Eur J Neurosci. 2000 Sep;12(9):3415-25
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7713126
|
|
Gene symbol:
|
Tac1
|
|
Gene name:
|
tachykinin 1
|
|
Probe:
|
substance P probe
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
1D SP DAT+/+:
embryonic day 13.0
(more  )
Specimen
1D SP DAT+/+:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: corpus striatum |
Present |
|
1D SP DAT+/+
|
Expression in striatal anlage.
|
Specimen
1D SP DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: corpus striatum |
Present |
|
1D SP DAT-/-
|
Expression in striatal anlage.
|
Specimen
SP G14 not shown:
embryonic day 14.0
(more )
Specimen
SP G14 not shown:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
Graded |
|
Expression heterogeneously distributed throughout striatum, with increasing ventrodorsal and mediolateral gradient of expression.
|
Specimen
SP G16 not shown:
embryonic day 16.0
(more )
Specimen
SP G16 not shown:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Present |
Regionally restricted |
|
Expression heterogeneously distributed throughout striatum, with increasing ventrodorsal and mediolateral gradient of expression.
|
Specimen
2C SP DAT+/+:
embryonic day 17.0
(more )
Specimen
2C SP DAT+/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Present |
Graded |
2C SP DAT+/+
|
Expression heterogeneously distributed throughout striatum, with increasing ventrodorsal and mediolateral gradient of expression.
|
Specimen
2C SP DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Present |
|
2C SP DAT-/-
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP P0 DAT+/+:
postnatal day 0
(more )
Specimen
SP P0 DAT+/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Patchy |
|
Patchy expression in both genotypes at P0 and P2.
|
Specimen
SP P0 DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Patchy |
|
Patchy expression in both genotypes at P0 and P2.
|
Specimen
3C SP DAT+/+:
postnatal day 2
(more )
Specimen
3C SP DAT+/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Patchy |
3C SP DAT+/+
|
Patchy expression in both genotypes at P0 and P2.
|
Specimen
3C SP DAT-/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
Patchy |
3C SP DAT-/-
|
Patchy expression. Levels of expression unchanged compared to wild type.
|
Specimen
SP whole striatum G13 +/+:
embryonic day 13.0
(more )
Specimen
SP whole striatum G13 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP whole striatum G13 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: corpus striatum |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP whole striatum G14 +/+:
embryonic day 14.0
(more )
Specimen
SP whole striatum G14 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP whole striatum G14 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP whole striatum G16 +/+:
embryonic day 16.0
(more )
Specimen
SP whole striatum G16 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP whole striatum G16 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: corpus striatum |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP whole striatum G17 +/+:
embryonic day 17.0
(more )
Specimen
SP whole striatum G17 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP whole striatum G17 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: corpus striatum |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP caudate-putamen P0 +/+:
postnatal day 0
(more )
Specimen
SP caudate-putamen P0 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP caudate-putamen P0 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: caudate-putamen |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP caudate-putamen P2 +/+:
postnatal day 2
(more )
Specimen
SP caudate-putamen P2 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP caudate-putamen P2 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: caudate-putamen |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP caudate-putamen P7 +/+:
postnatal day 7
(more )
Specimen
SP caudate-putamen P7 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP caudate-putamen P7 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: caudate-putamen |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP caudate-putamen Adult +/+:
postnatal adult
(more )
Specimen
SP caudate-putamen Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP caudate-putamen Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: caudate-putamen |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP nucleus accumbens P0 +/+:
postnatal day 0
(more )
Specimen
SP nucleus accumbens P0 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP nucleus accumbens P0 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: accumbens nucleus |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP nucleus accumbens P2 +/+:
postnatal day 2
(more )
Specimen
SP nucleus accumbens P2 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP nucleus accumbens P2 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: accumbens nucleus |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP nucleus accumbens P7 +/+:
postnatal day 7
(more )
Specimen
SP nucleus accumbens P7 +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP nucleus accumbens P7 -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: accumbens nucleus |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
Specimen
SP nucleus accumbens Adult +/+:
postnatal adult
(more )
Specimen
SP nucleus accumbens Adult +/+:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
SP nucleus accumbens Adult -/-:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Slc6a3tm1Mca/Slc6a3tm1Mca
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: accumbens nucleus |
Present |
|
|
Levels of expression unchanged compared to wild type.
|
|
Analysis Tools