Gene Expression Data
Assay Details
Assay
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Reference:
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J:131378
Liu H, et al., NeuroD1 regulates expression of thyroid hormone receptor 2 and cone opsins in the developing mouse retina. J Neurosci. 2008 Jan 16;28(3):749-56
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7713097
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Gene symbol:
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Opn1sw
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Gene name:
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opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan)
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Antibody:
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Anti-Blue opsin (AB5407)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The authors did not specify which antibody was used, MGI:7712878 may have been used instead.
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Results
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Specimen
2A:
postnatal day 14
(more )
Note: Flat mount. Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount. Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal cone cell |
Present |
Non-Uniform |
2A
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Expression is predominantly in cones in the dorsal retina.
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Specimen
2B:
postnatal day 14
(more )
Note: Flat mount. Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount. Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal cone cell |
Present |
Non-Uniform |
2B
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Expression is predominantly in cones in the dorsal retina.
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Specimen
2E:
postnatal day 14; Neurod1tm1Mjts/ Neurod1tm1Mjts
(more )
Note: Flat mount. Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
2E:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount. Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
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Specimen
2G:
postnatal day 14; Neurod1tm1Mjts/ Neurod1tm1Mjts
(more )
Note: Flat mount. Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
2G:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount. Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
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Specimen
3D:
embryonic day 18.0
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina layer |
retinal cone cell |
Present |
Single cells |
3D
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Expression is in a few scattered expressing cones.
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Specimen
3E:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina layer |
retinal cone cell |
Strong |
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3E
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Expression is in cone cells.
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Specimen
S1A:
postnatal day 12-16
(more )
Note: Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
S1A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 12-16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal cone cell |
Present |
Non-Uniform |
S1A
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Expression is predominantly in cones in the dorsal retina.
|
Specimen
S1B:
postnatal day 12-16
(more )
Note: Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
S1B:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 12-16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal cone cell |
Present |
Non-Uniform |
S1B
|
Expression is predominantly in cones in the dorsal retina.
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Specimen
S1E:
postnatal day 12-16; Neurod1tm1Mjts/ Neurod1tm1Mjts
(more )
Note: Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
S1E:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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postnatal day 12-16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dorsal retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
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Specimen
S1F:
postnatal day 12-16; Neurod1tm1Mjts/ Neurod1tm1Mjts
(more )
Note: Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
Specimen
S1F:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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postnatal day 12-16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Ventral retina. Double labeled: red - Opn1sw; green - peanut lectin agglutinin.
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