Bmp4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:106811 Johnson EB, et al., Abnormal development of the apical ectodermal ridge and polysyndactyly in Megf7-deficient mice. Hum Mol Genet. 2005 Nov 15;14(22):3523-38
Assay type:	RNA in situ
MGI Accession ID:	MGI:7712824
Gene symbol:	Bmp4
Gene name:	bone morphogenetic protein 4

Probe:	Bmp4 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 8A Forelimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		8A F WT

Specimen 8A Hindlimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		8A H WT

Specimen 8A Forelimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud ectoderm	Present	Regionally restricted	8A F KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: footplate apical ectodermal ridge	Weak	Non-Uniform	8A F KO

Specimen 8A Hindlimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud ectoderm	Present	Regionally restricted	8A H KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: footplate apical ectodermal ridge	Weak	Non-Uniform	8A H KO

Specimen 8B Forelimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		8B F WT

Specimen 8B Hindlimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		8B H WT

Specimen 8B Forelimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud ectoderm	Present	Regionally restricted	8B F KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: handplate apical ectodermal ridge	Weak	Non-Uniform	8B F KO

Specimen 8B Hindlimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud ectoderm	Present	Regionally restricted	8B H KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: footplate apical ectodermal ridge	Weak	Non-Uniform	8B H KO

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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