Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106811
Johnson EB, et al., Abnormal development of the apical ectodermal ridge and polysyndactyly in Megf7-deficient mice. Hum Mol Genet. 2005 Nov 15;14(22):3523-38
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7712793
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A Forelimb WT:
embryonic day 10.5
(more  )
Note: Wild type was either +/+ or heterozygous.
Specimen
6A Forelimb WT:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Wild type was either +/+ or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
6A F WT
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Expression is in the posterior mesoderm in dorsal and ventral patches.
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Specimen
6A Hindlimb WT:
embryonic day 10.5
(more )
Note: Wild type was either +/+ or heterozygous.
Specimen
6A Hindlimb WT:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Wild type was either +/+ or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6A H WT
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Expression is in the posterior mesoderm in dorsal and ventral patches.
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Specimen
6A Forelimb KO:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Lrp4tm1Her/Lrp4tm1Her
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
6A F KO
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In this mutant the expression patches are broadened and more diffuse compared to controls with the dorsal patch expressing higher levels than the ventral one.
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Specimen
6A Hindlimb KO:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Lrp4tm1Her/Lrp4tm1Her
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6A H KO
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In this mutant the expression patches are broadened and more diffuse compared to controls with the dorsal patch expressing higher levels than the ventral one.
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Specimen
6B Forelimb WT:
embryonic day 11.5
(more )
Note: Wild type was either +/+ or heterozygous.
Specimen
6B Forelimb WT:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Wild type was either +/+ or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
6B F WT
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Expression is in the posterior mesoderm in dorsal and ventral patches.
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Specimen
6B Hindlimb WT:
embryonic day 11.5
(more )
Note: Wild type was either +/+ or heterozygous.
Specimen
6B Hindlimb WT:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Wild type was either +/+ or heterozygous.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6B H WT
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Expression is in the posterior mesoderm in dorsal and ventral patches.
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Specimen
6B Forelimb KO:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Lrp4tm1Her/Lrp4tm1Her
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
6B F KO
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In this mutant the expression patches are broadened and more diffuse compared to controls with the dorsal patch expressing higher levels than the ventral one.
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Specimen
6B Hindlimb KO:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Lrp4tm1Her/Lrp4tm1Her
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6B H KO
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In this mutant the expression patches are broadened and more diffuse compared to controls with the dorsal patch expressing higher levels than the ventral one.
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Analysis Tools