Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:106811 Johnson EB, et al., Abnormal development of the apical ectodermal ridge and polysyndactyly in Megf7-deficient mice. Hum Mol Genet. 2005 Nov 15;14(22):3523-38
Assay type:	RNA in situ
MGI Accession ID:	MGI:7712780
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 5A WT: embryonic day 9.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		5A WT

Specimen 5A KO: embryonic day 9.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		5A KO
TS15: forelimb bud ectoderm	Present	Regionally restricted	5A KO	There is diffuse expression over the entire distal end of the developing limb bud.

Specimen 5B Forelimb WT: embryonic day 10.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		5B F WT

Specimen 5B Hindlimb WT: embryonic day 10.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present		5B H WT

Specimen 5B Forelimb KO: embryonic day 10.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present	Regionally restricted	5B F KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS17: forelimb bud apical ectodermal ridge	Present	Non-Uniform	5B F KO

Specimen 5B Hindlimb KO: embryonic day 10.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present	Regionally restricted	5B H KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS17: hindlimb bud apical ectodermal ridge	Present	Non-Uniform	5B H KO

Specimen 5C Forelimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		5C F WT

Specimen 5C Hindlimb WT: embryonic day 11.5 (more

)
Note: Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		5C H WT

Specimen 5C Forelimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud ectoderm	Present	Regionally restricted	5C F KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: handplate apical ectodermal ridge	Present	Non-Uniform	5C F KO

Specimen 5C Hindlimb KO: embryonic day 11.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud ectoderm	Present	Regionally restricted	5C H KO	Expression is split into a dorsal and a ventral ridge of high expression that is separated by an area of lower and diffuse expression.
TS19: footplate apical ectodermal ridge	Present	Non-Uniform	5C H KO

Specimen 5D WT: embryonic day 10.5 (more

)
Note: Vibratome crosssection. Wild type was either +/+ or heterozygous.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		5D WT

Specimen 5D KO: embryonic day 10.5; Lrp4^tm1Her/Lrp4^tm1Her (more

)
Note: Vibratome crosssection.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present	Regionally restricted	5D KO	There is diffuse expression over the entire distal end of the developing limb bud.
TS17: forelimb bud apical ectodermal ridge	Present		5D KO

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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