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Gene Expression Data
Assay Details
Assay
Reference: J:62565 Cowan CA, et al., EphB2 guides axons at the midline and is necessary for normal vestibular function. Neuron. 2000 May;26(2):417-30
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7711676
Gene symbol: Ephb2
Gene name: Eph receptor B2
Reporter: lacZ
Direct Detection
Results
Specimen 1A: embryonic day 12.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS20: vestibulo-cochlear ganglion Strong Regionally restricted 1A Strong expression in vesibulo-acoustic VIII ganglion, including axons extending toward posterior vertical canal.
TS20: facial nerve Present Regionally restricted 1A Expression in axons of facial (VII) nerve.

Specimen 1C: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 1C Expression in epithelial cells immediately adjacent to sensory epithelium in semicircular canals including horizontal canal.

Specimen 1D: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: cochlear ganglion Present 1D
TS22: saccule Present 1D

Specimen 1E: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: saccule Present 1E
TS22: vestibular ganglion Present 1E
TS22: utricle Present Regionally restricted 1E Expression in epithelial cells immediately adjacent to sensory epithelium of macula of utricle.

Specimen 1F: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: cochlear ganglion Present 1F Expression in spiral ganglion.
TS22: vestibular ganglion Present 1F
TS22: cochlear duct Present Regionally restricted 1F Expression in stria vascularis.

Specimen 1G: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: lateral semicircular canal epithelium Present Regionally restricted 1G Expression in nonsensory epithelial cells adjacent to sensory epithelia in crista of semicircular canals.

Specimen 1H: embryonic day 14.5; Ephb2tm2Paw/Ephb2+ (more )
Structure Level Pattern Image Note
TS22: cochlear ganglion Present Regionally restricted 1H Expression in spiral ganglion. Expression in axons of spiral ganglion extending into organ of Corti.
TS22: cochlear duct Present Regionally restricted 1H Expression in stria vascularis.

Specimen 7A,B: postnatal day 21; Ephb2tm2Paw/Ephb2+ (more )
Structure Cell Type Level Pattern Image Note
TS28: inner ear vestibular component vestibular dark cell Strong Regionally restricted 7A , 7B High levels of expression in patches of dark cells bordering cristae in ampulae and macula of utricle.
TS28: crista ampullaris Absent 7B , 7A
TS28: macula of utricle Absent 7B , 7A

Specimen 7C: postnatal day 21; Ephb2tm2Paw/Ephb2+ (more )
Structure Cell Type Level Pattern Image Note
TS28: inner ear vestibular component vestibular dark cell Present Regionally restricted 7C Expression in single-cell layer of dark cells within vestibular epithelium.
TS28: crista ampullaris Absent 7C No expression in adjacent sensory epithelium in macula of utricle or crista of anterior vertical canal.
TS28: macula of utricle Absent 7C No expression in adjacent sensory epithelium in macula of utricle or crista of anterior vertical canal.

Specimen 7D: postnatal day 21; Ephb2tm2Paw/Ephb2+ (more )
Structure Cell Type Level Pattern Image Note
TS28: inner ear vestibular component vestibular dark cell Present 7D Expression specific to vestibular dark cells.
TS28: crista ampullaris Absent 7D No expression in transitional cells, hair cells, or support cells of crista.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory