Ascl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:112208 Fotaki V, et al., Abnormal positioning of diencephalic cell types in neocortical tissue in the dorsal telencephalon of mice lacking functional Gli3. J Neurosci. 2006 Sep 6;26(36):9282-92
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7711123
Gene symbol:	Ascl1
Gene name:	achaete-scute family bHLH transcription factor 1

Antibody:	anti-Mash1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

2E ©

Specimen 2E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2E	Expression in this mutant is similar to wild-type.
TS20: ganglionic eminence	Present		2E	Expression in this mutant is similar to wild-type.
TS20: hypothalamus	Present		2E	Expression in this mutant is similar to wild-type.
TS20: thalamus	Present	Regionally restricted	2E	Expression is ventral. Expression in this mutant is similar to wild-type.

2E' ©

Specimen 2E': embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2E'
TS20: ganglionic eminence	Present		2E'
TS20: hypothalamus	Present		2E'
TS20: thalamus	Present	Regionally restricted	2E'	Expression is ventral.

2F ©

Specimen 2F: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2F
TS20: ganglionic eminence	Present		2F
TS20: hypothalamus	Present		2F
TS20: thalamus	Present	Regionally restricted	2F	Expression is ventral.

2F' ©

Specimen 2F': embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2F'	Expression in this mutant is similar to wild-type.
TS20: ganglionic eminence	Present		2F'	Expression in this mutant is similar to wild-type.
TS20: hypothalamus	Present		2F'	Expression in this mutant is similar to wild-type.
TS20: thalamus	Present	Regionally restricted	2F'	Expression is ventral. Expression in this mutant is similar to wild-type.

2G ©

Specimen 2G: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2G
TS20: ganglionic eminence	Present		2G
TS20: hypothalamus	Present		2G
TS20: thalamus	Present	Regionally restricted	2G	Expression is ventral.
TS20: neocortex	Absent		2G

2G' ©

Specimen 2G': embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present		2G'
TS20: ganglionic eminence	Present		2G'
TS20: hypothalamus	Present		2G'
TS20: thalamus	Present	Regionally restricted	2G'	Expression is ventral.
TS20: neocortex	Absent		2G'

2H ©

Specimen 2H: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present		2H
TS20: neocortex	Absent		2H

2H' ©

Specimen 2H': embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present		2H'
TS20: neocortex	Absent		2H'

4B ©

Specimen 4B: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present		4B
TS21: hypothalamus	Present	Regionally restricted	4B	Expression is ventral.
TS21: telencephalon	Present	Regionally restricted	4B	Expression is ventral.
TS21: thalamus	Present	Regionally restricted	4B	Expression is ventral.

4B' ©

Specimen 4B': embryonic day 13.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	4B'	Expression in this mutant is in a similar region as seen in wild-type.
TS21: telencephalon	Present	Regionally restricted	4B'	Expression in this mutant is in a similar region as seen in wild-type.

Specimen not shown WT E14.5: embryonic day 14.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted		Expression is ventral.
TS22: thalamus	Present	Regionally restricted		Expression is ventral.

Specimen not shown Gli3^Xt E14.5: embryonic day 14.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted		Expression is ventral.
TS22: thalamus	Present	Regionally restricted		Expression is ventral.

Specimen not shown WT E15.5: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Regionally restricted		Expression is ventral.
TS23: thalamus	Present	Regionally restricted		Expression is ventral.

Specimen not shown Gli3^Xt E15.5: embryonic day 15.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Regionally restricted		Expression is ventral.
TS23: thalamus	Present	Regionally restricted		Expression is ventral.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24