Irx1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163793 McDonald LA, et al., Comparison of Iroquois gene expression in limbs/fins of vertebrate embryos. J Anat. 2010 Jun;216(6):683-91
Assay type:	RNA in situ
MGI Accession ID:	MGI:7709789
Gene symbol:	Irx1
Gene name:	Iroquois homeobox 1

Probe:	IMAGE clone 2615958
Probe preparation:	Antisense RNA

Results

Specimen 2/S2A fl: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2A fl , S2A fl	Expressed in the proximal regions.

Specimen 2/S2A hl: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	2A hl , S2A hl	Expressed in the proximal regions.

Specimen 2/S2B fl: embryonic day 12.5 (more

)

Structure	Level	Image	Note
TS20: forelimb digit	Present	2B fl , S2B fl	Expressed in condensing digits, with stronger expression in digits 2-4.
TS20: forelimb digit 2	Present	S2B fl , 2B fl	Expression was seen first and was strongest.
TS20: forelimb digit 3	Present	S2B fl , 2B fl	Expression was seen first and was strongest.
TS20: forelimb digit 4	Present	S2B fl , 2B fl	Expression was seen first and was strongest.
TS20: forelimb digit 1	Present	S2B fl , 2B fl	Weaker expression.
TS20: forelimb digit 5	Present	S2B fl , 2B fl	Weaker expression.

Specimen 2/S2B hl: embryonic day 12.5 (more

)

Structure	Level	Image	Note
TS20: hindlimb digit	Present	S2B hl , 2B hl	Expressed in condensing digits, with stronger expression in digits 2-4.
TS20: hindlimb digit 2	Present	S2B hl , 2B hl	Stronger expression.
TS20: hindlimb digit 3	Present	S2B hl , 2B hl	Stronger expression.
TS20: hindlimb digit 4	Present	S2B hl , 2B hl	Stronger expression.
TS20: hindlimb digit 1	Present	2B hl , S2B hl	Weaker expression.
TS20: hindlimb digit 5	Present	2B hl , S2B hl	Weaker expression.

Specimen 2/S2C fl: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: hand joint	Present	Regionally restricted	S2C fl , 2C fl	Expression was stronger in the digits 2, 3 and 4.
TS21: forelimb digit 2	Present	Regionally restricted	S2C fl , 2C fl	Expression was stronger in the joint-forming regions.
TS21: forelimb digit 3	Present	Regionally restricted	S2C fl , 2C fl	Expression was stronger in the joint-forming regions.
TS21: forelimb digit 4	Present	Regionally restricted	S2C fl , 2C fl	Expression was stronger in the joint-forming regions.

Specimen 2/S2C hl: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: foot joint	Present	Regionally restricted	2C hl , S2C hl	Expression was stronger in the digits 2, 3 and 4.
TS21: hindlimb digit 2	Present	Regionally restricted	2C hl , S2C hl	Expression was stronger in the joint-forming regions.
TS21: hindlimb digit 3	Present	Regionally restricted	2C hl , S2C hl	Expression was stronger in the joint-forming regions.
TS21: hindlimb digit 4	Present	Regionally restricted	2C hl , S2C hl	Expression was stronger in the joint-forming regions.

Specimen 2/S2D fl: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hand joint	Present	Regionally restricted	S2D fl , 2D fl	Expressed in joint-forming regions in all digits.

Specimen 2/S2D hl: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: foot joint	Present	Regionally restricted	2D hl , S2D hl	Expressed in joint-forming regions in all digits.

Specimen 4C FL: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Very strong	Widespread	4C fl	Expressed in all digit condensations.

Specimen 4C HL: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit	Very strong	Widespread	4C hl	Expressed in all digit condensations.

Specimen 4D FL: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: hand joint	Present	Regionally restricted	4D fl	Expressed in joint-forming regions in all the digits.
TS21: forelimb digit 1	Present	Regionally restricted	4D fl	Expressed in joint-forming regions.
TS21: forelimb digit 2	Present	Regionally restricted	4D fl	Expressed in joint-forming regions.
TS21: forelimb digit 3	Present	Regionally restricted	4D fl	Expressed in joint-forming regions.
TS21: forelimb digit 4	Present	Regionally restricted	4D fl	Expressed in joint-forming regions.
TS21: forelimb digit 5	Present	Regionally restricted	4D fl	Expressed in joint-forming regions.

Specimen 4D HL: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 1	Strong		4D hl	Expressed throughout the skeleton of the digit.
TS21: hindlimb digit 5	Strong		4D hl	Expressed throughout the skeleton of the digit.
TS21: hindlimb digit 2	Present	Regionally restricted	4D hl	Expressed in joint-forming regions.
TS21: hindlimb digit 3	Present	Regionally restricted	4D hl	Expressed in joint-forming regions.
TS21: hindlimb digit 4	Present	Regionally restricted	4D hl	Expressed in joint-forming regions.
TS21: foot joint	Present	Regionally restricted	4D hl	Expressed in joint-forming regions of digits 2, 3 and 4.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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