Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:55694
Bermingham NA, et al., Math1: an essential gene for the generation of inner ear hair cells. Science. 1999 Jun 11;284(5421):1837-41
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:7709752
|
|
Gene symbol:
|
Atoh1
|
|
Gene name:
|
atonal bHLH transcription factor 1
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1B:
embryonic day 12.5; Atoh1tm2Hzo/Atoh1 +
(more  )
Specimen
1B:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: inner ear |
Present |
Regionally restricted |
1B
|
Expression detected throughout prospective sensory epithelium of otic vesicle before differentiation of inner ear hair cells.
|
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1tm2Hzo
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: inner ear |
Present |
Regionally restricted |
1C
|
Expression detected throughout prospective sensory epithelium of otic vesicle before differentiation of inner ear hair cells.
|
Specimen
1D:
embryonic day 14.5; Atoh1tm2Hzo/Atoh1 +
(more )
Specimen
1D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Widespread |
1D
|
Expression throughout epithelia of utricle, saccule, semicircular canals and cochlea.
|
| TS22: saccule |
Present |
Widespread |
1D
|
Expression throughout epithelia of utricle, saccule, semicircular canals and cochlea.
|
| TS22: semicircular canal |
Present |
Widespread |
1D
|
Expression throughout epithelia of utricle, saccule, semicircular canals and cochlea.
|
| TS22: utricle |
Present |
Widespread |
1D
|
Expression throughout epithelia of utricle, saccule, semicircular canals and cochlea.
|
Specimen
1E:
embryonic day 18.5; Atoh1tm2Hzo/Atoh1 +
(more )
Specimen
1E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
cochlear inner hair cell |
Present |
Regionally restricted |
1E
|
Expression restricted to hair cells. Expression in one inner row and 3 outer rows of hair cell.
|
| TS26: cochlea |
cochlear outer hair cell |
Present |
Regionally restricted |
1E
|
Expression restricted to hair cells. Expression in one inner row and 3 outer rows of hair cell.
|
Specimen
1F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1tm2Hzo
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Trace |
Regionally restricted |
1F
|
Expression greatly decreased in middle turn of cochlea. Expression in some supporting cell layers.
|
Specimen
1G:
embryonic day 18.5; Atoh1tm2Hzo/Atoh1 +
(more )
Specimen
1G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
1G inset
,
1G
|
In basal region of cochlea, expression restricted to hair cells
|
Specimen
1H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1tm2Hzo
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Trace |
Regionally restricted |
1H inset
,
1H
|
Expression absent in basal turn. Hair cells are absent.
|
|
Analysis Tools
