Antibody:	Anti-HNF-4 alpha (C-19, sc-6556)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: liver	hepatocyte	Present		2a	Expression was detected in the nuclei of hepatocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	intestinal epithelial cell	Present	Regionally restricted	2b	In the intestines, the immunoreaction in the nuclei of crypt epithelium was stronger than those in villous or superficial epithelium.
TS28: intestinal villus	intestinal epithelial cell	Present	Regionally restricted	2b	In the intestines, the immunoreaction in the nuclei of crypt epithelium was stronger than those in villous or superficial epithelium.
TS28: intestine epithelium	intestinal epithelial cell	Present	Regionally restricted	2b	In the intestines, the immunoreaction in the nuclei of crypt epithelium was stronger than those in villous or superficial epithelium.

Structure	Level	Pattern	Image	Note
TS28: stomach	Weak		2c

Structure	Level	Pattern	Image	Note
TS28: pancreas	Absent		2d

Structure	Cell Type	Level	Pattern	Image	Note
TS28: proximal straight tubule, cortical portion	epithelial cell of proximal tubule	Present	Regionally restricted	3a	In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. Immunohistochemistry showed a positive reaction in the neck of the proximal tubule.
TS28: renal proximal convoluted tubule	epithelial cell of proximal tubule	Present	Regionally restricted	3a	In the cortex, expression was detected in the nuclei of epithelial cells constructing the convoluted and straight proximal tubules. Immunohistochemistry showed a positive reaction in the neck of the proximal tubule.
TS28: glomerular tuft		Absent		3a

Structure	Level	Pattern	Image	Note
TS28: cortical renal tubule	Present	Regionally restricted	3b	In the boundary region between the kidney cortex and medulla, the positive reaction disappeared suddenly at the attenuated tubules.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: stomach epithelium	epithelial cell of stomach	Present		4a

Structure	Cell Type	Level	Pattern	Image	Note
TS20: anal region	intestinal epithelial cell	Present	Regionally restricted	4b	In the rectoanal region of the alimentary tract, immunoreactions of cell nuclei constructing the stratified columnar contour gradually disappeared towards the ectoderm.
TS20: hindgut epithelium	intestinal epithelial cell	Present	Regionally restricted	4b	In the rectoanal region of the alimentary tract, immunoreactions of cell nuclei constructing the stratified columnar contour gradually disappeared towards the ectoderm.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pancreas	pancreatic acinar cell	Present		4c

Structure	Cell Type	Level	Pattern	Image	Note
TS20: mesonephric tubule	epithelial cell	Present		4d
TS20: nephric duct, mesonephric portion		Absent		4d

Structure	Level	Pattern	Image	Note
TS20: liver	Present

Structure	Level	Pattern	Image	Note
TS22: renal vesicle	Absent		5a	The round metanephric vesicles were negative.
TS22: nephrogenic interstitium	Absent		5a	Newly formed blastema were negative.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: comma-shaped body	epithelial cell of nephron	Present	Regionally restricted	5b	Some epithelial cells in comma-shaped bodies were positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: s-shaped body	epithelial cell of nephron	Present	Regionally restricted	5c	Some epithelial cells in S-shaped bodies were positive.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: s-shaped body	epithelial cell of nephron	Present	Regionally restricted	5d	Some epithelial cells in S-shaped bodies were positive. Positive reaction was detected on the opposite area of capillary invasion in the elongating S-shaped body.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	metanephric podocyte	Present	Regionally restricted	5e	In the primitive glomerulus, podocytes were immunopositive.

Structure	Level	Pattern	Image	Note
TS24: nephron epithelium	Present	Regionally restricted	7b	Expression was detected in the late nephrogenic epithelial tissues.
TS24: comma-shaped body	Weak		7b
TS24: s-shaped body	Weak		7b