Col2a1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:45403 Shibata S, et al., Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible. J Anat. 1997 Nov;191(Pt 4):561-70
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7709196
Gene symbol:	Col2a1
Gene name:	collagen, type II, alpha 1

Antibody:	anti-Col2a1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 1b: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: radius-ulna cartilage condensation	Present	Widespread	1b	Expressed throughout the cartilage matrix, especially extensive in the diaphysis.

Specimen 2b: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: ulna cartilage condensation	Present	Widespread	2b	Expressed throughout the cartilage.

Specimen 3b: embryonic day 14.5 (more

)

Structure	Level	Image
TS22: mandible condylar condensation	Absent	3b
TS22: Meckel's cartilage	Present	3b
TS22: sphenoid bone	Present	3b
TS22: temporal bone	Present	3b

Specimen 4c: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: mandible condylar process	Present	Regionally restricted	4c	Expressed around the hypertrophic chondrocytes.
TS23: Meckel's cartilage	Present		4c
TS23: sphenoid bone	Present		4c
TS23: temporal bone	Present		4c

Specimen 4d: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: mandible condylar process	Present	Regionally restricted	4d	Expressed around the hypertrophic chondrocytes.

Specimen 6b: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: mandible condylar process	Present	Regionally restricted	6b	Expressed both in the hypertrophic cell and flattened cell zones.
TS24: temporal bone	Present		6b

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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