About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:76140 Shibata S, et al., In situ hybridization and immunohistochemistry of bone sialoprotein and secreted phosphoprotein 1 (osteopontin) in the developing mouse mandibular condylar cartilage compared with limb bud cartilage. J Anat. 2002 Mar;200(Pt 3):309-20
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7707668
Gene symbol: Spp1
Gene name: secreted phosphoprotein 1
Antibody: anti-Spp1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen not shown: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: tibia-fibula pre-cartilage condensation Absent Expression not detected at this stage.
Specimen 2H: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: bone Present Regionally restricted 2H Expression in bone callar.
TS22: tibia cartilage condensation Present Regionally restricted 2H Expression in matrix of lower hypertrophic cell zone.
Specimen 3F: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: tibia epiphysis Present Regionally restricted 3F Expression in bone collar.
TS23: epiphyseal plate hypertrophic zone Present Regionally restricted 3F Expression more extensive in lower hypertrophic cells zone.
Specimen 5F: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: mandible Present Regionally restricted 5F Expression in bone collar.
TS23: mandible condylar process Absent 5F No expression in newly formed cartilage matrix.
Specimen 6F: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: mandible Present Regionally restricted 6F Expression in bone collar.
TS24: mandible condylar process Present Regionally restricted 6F Expression in entire hypertrophic zone.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory