Gene Expression Data
Assay Details
Assay
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Reference:
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J:76140
Shibata S, et al., In situ hybridization and immunohistochemistry of bone sialoprotein and secreted phosphoprotein 1 (osteopontin) in the developing mouse mandibular condylar cartilage compared with limb bud cartilage. J Anat. 2002 Mar;200(Pt 3):309-20
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7707666
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Gene symbol:
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Col10a1
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Gene name:
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collagen, type X, alpha 1
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Probe:
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Col10a1 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2F:
embryonic day 14.5
(more )
Specimen
2F:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: tibia cartilage condensation |
chondrocyte |
Present |
Regionally restricted |
2F
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Expression in chondrocytes of entire hypertrophic cell zone.
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Specimen
3D:
embryonic day 15.5
(more )
Specimen
3D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: epiphyseal plate hypertrophic zone |
Present |
Regionally restricted |
3D
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Expression in peripheral part of hypertrophic cell zone corresponding to upper hypertrophic cell zone. Expression less extensive in lower hypertrophic cell zone.
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TS23: tibia epiphysis |
Present |
Regionally restricted |
3D
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Expression in peripheral part of hypertrophic cell zone corresponding to upper hypertrophic cell zone. Expression less extensive in lower hypertrophic cell zone.
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Specimen
4D:
embryonic day 14.5
(more )
Specimen
4D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5D:
embryonic day 15.5
(more )
Specimen
5D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: mandible condylar process |
chondrocyte |
Present |
Widespread |
5D
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Widely expressed in newly formed chondrocytes.
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Specimen
6D:
embryonic day 16.5
(more )
Specimen
6D:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: mandible condylar process |
chondrocyte |
Present |
Regionally restricted |
6D
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Expression in chondrocytes of upper hypertrophic zone. Weak expression in lower hypertrophic zone.
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