Antibody:	anti-PTEN
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	strial marginal cell	Present		2a , 2c	Expressed in the differentiating cells, showing a clear cytoplasmic staining.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: stria vascularis	strial marginal cell	Present		2d , 2f	Expression was much lower, although the cytoplasmic staining pattern was similar to that at E17.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: stria vascularis	strial marginal cell	Absent		3a , 3c
TS27: stria vascularis		Present	Regionally restricted	3c , 3a	Relatively high expressed in the root cells and root-like processes. Not expressed in marginal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spiral ligament	type 1 otic fibrocyte	Present		3d , 3f
TS28: spiral ligament	type 2 otic fibrocyte	Present		3d , 3f
TS28: spiral ligament	type 3 otic fibrocyte	Present		3d , 3f
TS28: spiral ligament	type 4 otic fibrocyte	Present		3d , 3f
TS28: spiral ligament	type 5 otic fibrocyte	Present		3d , 3f
TS28: stria vascularis		Present	Regionally restricted	3d , 3f	Expressed in the root cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spiral ligament	type 1 otic fibrocyte	Present		4c , 4a	Expression was much lower than that of P10.
TS28: spiral ligament	type 2 otic fibrocyte	Present		4c , 4a	Expression was much lower than that of P10.
TS28: spiral ligament	type 3 otic fibrocyte	Present		4c , 4a	Expression was much lower than that of P10.
TS28: spiral ligament	type 4 otic fibrocyte	Present		4c , 4a	Expression was much lower than that of P10.
TS28: spiral ligament	type 5 otic fibrocyte	Present		4c , 4a	Expression was much lower than that of P10.
TS28: stria vascularis		Present	Regionally restricted	4c , 4a	Remained high expression in the root cells and root-like processes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spiral ligament	type 1 otic fibrocyte	Absent		4f , 4d
TS28: spiral ligament	type 2 otic fibrocyte	Absent		4f , 4d
TS28: spiral ligament	type 3 otic fibrocyte	Absent		4f , 4d
TS28: spiral ligament	type 4 otic fibrocyte	Absent		4f , 4d
TS28: spiral ligament	type 5 otic fibrocyte	Absent		4f , 4d
TS28: stria vascularis		Present	Regionally restricted	4d , 4f	Remained high expression in the root cells and root-like processes, showing the morphological characteristic of finger-like basolateral projection.