Gene Expression Data
Assay Details
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Assay
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Reference:
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J:275368
Shu P, et al., The spatiotemporal expression pattern of microRNAs in the developing mouse nervous system. J Biol Chem. 2019 Mar 8;294(10):3444-3453
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7707444
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Gene symbol:
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Mir218-2
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Gene name:
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microRNA 218-2
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Probe:
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mmu-miR-218 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
5A:
embryonic day 10.0-10.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.0-10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B:
embryonic day 11.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
spinal cord motor neuron |
Present |
Regionally restricted |
5C
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Expressed in the ventrolateral spinal cord, where motor neurons reside.
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Specimen
5D:
embryonic day 13.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
spinal cord motor neuron |
Present |
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5D
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Specimen
5E/E':
embryonic day 11.5
(more )
Note: Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
Specimen
5E/E':
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5E'
,
5E
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Most of the positive cells co-expressed Islet-1/2.
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Specimen
5G:
embryonic day 11.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5G
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5H:
embryonic day 11.5
(more )
Specimen
5H:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5H
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5I:
embryonic day 11.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5I
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5J:
embryonic day 11.5
(more )
Specimen
5J:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5J
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5K:
embryonic day 11.5
(more )
Specimen
5K:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5K
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5L:
embryonic day 11.5
(more )
Specimen
5L:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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5L
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Detected in all subtypes of motor neurons from the medulla to the spinal cord, and in lateral
motor column and medial motor column spinal motor neurons from cervical to lumbar regions.
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Specimen
5M:
embryonic day 12.5
(more )
Specimen
5M:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brainstem nucleus |
Present |
Regionally restricted |
5M
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Expressed in the brainstem motor nucleus to the spinal cord motor neurons.
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Specimen
5N:
embryonic day 12.5
(more )
Specimen
5N:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brainstem nucleus |
Present |
Regionally restricted |
5N
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Expressed in the brainstem motor nucleus to the spinal cord motor neurons.
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Specimen
S5A:
embryonic day 11.5
(more )
Note: Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
Specimen
S5A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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S5A
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Most of the positive cells co-expressed Islet-1/2.
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Specimen
S5B:
embryonic day 11.5
(more )
Note: Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
Specimen
S5B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - Mir218-2 and Mir218-1; brown - Isl1 (assay MGI:7705961) and Isl2 (assay MGI:7707434).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
spinal cord motor neuron |
Present |
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S5B
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Most of the positive cells co-expressed Islet-1/2.
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Analysis Tools