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Gene Expression Data
Assay Details
Assay
Reference: J:73206 Bard JB, et al., Early nephron formation in the developing mouse kidney. J Anat. 2001 Oct;199(Pt 4):385-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7707370
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: clone NCAM-OB11 (C9672)
Detection system: Secondary antibody coupled to Cy2
Results
Specimen 1: embryonic day 12.5 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 1 Expression in cap of condensed metanephric mesenchyme.
Specimen 2a: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 2a Expression in cap of cells with almost columnar morphology.
Specimen 2b: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 2b Expression in aggregate of metanephric mesenchyme cells.
Specimen 2c: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 2c Expression in aggregate of metanephric mesenchyme cells.
Specimen 2d: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 2d Expression in aggregate of metanephric mesenchyme cells.
Specimen 3: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 3 Expression in condensed caps.
TS20: metanephros Present Regionally restricted 3 Expression in aggregates. Lower expression in intervening unaggregated metanephir mesencnyme.
Specimen 4: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 4 Expression in aggregate of metanephric mesenchyme cells.
Specimen 5a: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: early nephron Present 5a
Specimen 5b: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: early nephron Present Regionally restricted 5b Expression in region of nephron within duct.
Specimen 5c: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: early nephron Present Regionally restricted 5c Expression in primitive nephron extending from duct epithelium.
Specimen 5d: embryonic day 12.75 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS20: early nephron Present Regionally restricted 5d Expression in primitive nephron extending from duct epithelium.
Specimen 6: embryonic day 13.5 (more )
Note: Triple labeled: red - Ncam1; green - laminin; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS21: early nephron Present Regionally restricted 6 Expression in more mature aggregate fused to a duct.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory