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Gene Expression Data
Assay Details
Assay
Reference: J:63394 Clark RM, et al., A novel candidate gene for mouse and human preaxial polydactyly with altered expression in limbs of Hemimelic extra-toes mutant mice. Genomics. 2000 Jul 1;67(1):19-27
Assay type: Northern blot
MGI Accession ID: MGI:7707355
Gene symbol: Lmbr1
Gene name: limb region 1
Probe: Lmbr1 probe
Probe preparation: labelled with P32 DNA
Visualized with: Phosphorimaging
Results Image: 4a Lmbr1
 Sample Information Bands Other Sample Information
Lane AgeStructure 5 kb 3 kb Amount Genetic BackgroundMutant Allele(s)Sex
E11.0 hind limb +/+ E11.0 TS18: hindlimb bud Present Present 15 µg; total RNA CD-1 Not Specified
E11.0 hind limb Hx/Hx E11.0 TS18: hindlimb bud Present (a) Present (a) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
E11.0 fore limb +/+ E11.0 TS18: forelimb bud Present Present 15 µg; total RNA CD-1 Not Specified
E11.0 fore limb Hx/Hx E11.0 TS18: forelimb bud Present (a) Present (a) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
E11.5 hind limb +/+ E11.5 TS19: hindlimb bud Present Present 15 µg; total RNA CD-1 Not Specified
E11.5 hind limb Hx/Hx E11.5 TS19: hindlimb bud Absent (b) Absent (b) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
E11.5 fore limb +/+ E11.5 TS19: forelimb bud Present Present 15 µg; total RNA CD-1 Not Specified
E11.5 fore limb Hx/Hx E11.5 TS19: forelimb bud Present (c) Present (c) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
E12.0 hind limb +/+ E12.0 TS20: hindlimb Present Present 15 µg; total RNA CD-1 Not Specified
E12.0 hind limb Hx/Hx E12.0 TS20: hindlimb Absent (d) Absent (d) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
E12.0 fore limb +/+ E12.0 TS20: forelimb Present Present 15 µg; total RNA CD-1 Not Specified
E12.0 fore limb Hx/Hx E12.0 TS20: forelimb Absent (d) Absent (d) 15 µg; total RNA involves: C57BL/10SnJ * M. m. castaneus Rr29Hx/Rr29HxNot Specified
Notes:
(a) Expression at near wild type levels at E11.0.
(b) Expression dowregulated in hindlimbs; not detectable above background levels.
(c) Expression still present in forelimbs. Authors report expression may be upregulated.
(d) Expression dramatically dowregulated; not detectable above background levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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