Probe:	ST8Sia II probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		2_Antisense (upper)	Prominent expression in neocortex, hippocampus, subiculum, thalamus and inferior colliculus.
TS27: inferior colliculus	Strong		2_Antisense (upper)	Prominent expression in neocortex, hippocampus, subiculum, thalamus and inferior colliculus.
TS27: neocortex	Strong		2_Antisense (upper)	Prominent expression in neocortex, hippocampus, subiculum, thalamus and inferior colliculus.
TS27: subiculum	Strong		2_Antisense (upper)	Prominent expression in neocortex, hippocampus, subiculum, thalamus and inferior colliculus.
TS27: thalamus	Strong		2_Antisense (upper)	Prominent expression in neocortex, hippocampus, subiculum, thalamus and inferior colliculus.
TS27: cerebellum	Moderate		2_Antisense (upper)	Moderate levels of expression in piriform cortex, cerebellum and superior colliculus.
TS27: piriform cortex	Moderate		2_Antisense (upper)	Moderate levels of expression in piriform cortex, cerebellum and superior colliculus.
TS27: superior colliculus	Moderate		2_Antisense (upper)	Moderate levels of expression in piriform cortex, cerebellum and superior colliculus.

Structure	Level	Pattern	Image	Note
TS27: medial habenular nucleus	Strong		2_Antisense (lower left)	Prominent expression in medial habenular nucleus.
TS27: piriform cortex	Moderate		2_Antisense (lower left)	Moderate levels of expression in piriform cortex.

Structure	Level	Pattern	Image	Note
TS27: brain	Present		2_Antisense (lower right)

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Very strong		3A	Strong expression in cortical plate at E16.
TS24: thalamus	Very strong	Widespread	3A	Strong widespread expression in thalamus from E16 to P3

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Strong
TS24: hippocampus	Weak

Structure	Level	Pattern	Image	Note
TS27: neocortex	Very strong	Widespread	3C	Widely expressed throughout developing neocortex.
TS27: thalamus	Strong	Widespread	3C	Strong widespread expression in thalamus from E16 to P3.
TS27: hippocampus pyramidal cell layer	Strong		3C	Abundantly expressed in pyramidal cell layer.
TS27: inferior colliculus	Strong		3C	Intense expression in inferior colliculus and pontine nuclei, strongest from P1 to P3.
TS27: pontine nucleus	Strong		3C	Intense expression in inferior colliculus and pontine nuclei, strongest from P1 to P3.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Very strong	Widespread	3D	Widely expressed throughout developing neocortex.
TS27: thalamus	Strong	Widespread	3D	Strong widespread expression in thalamus from E16 to P3.
TS27: hippocampus pyramidal cell layer	Strong		3D	Abundantly expressed in pyramidal cell layer.
TS27: inferior colliculus	Strong		3D	Intense expression in inferior colliculus and pontine nuclei, strongest from P1 to P3.
TS27: pontine nucleus	Strong		3D	Intense expression in inferior colliculus and pontine nuclei, strongest from P1 to P3.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Moderate	Regionally restricted	3E	Regional expression in neocortex occurred from P5 onwards.
TS28: frontal cortex	Absent		3E	Expression disappeared most prominently from frontal cortex.
TS28: hippocampus pyramidal cell layer	Strong		3E	Abundantly expressed in pyramidal cell layer.
TS28: inferior colliculus	Present		3E	Expression gradually decreased to P14.
TS28: pontine nucleus	Present		3E	Expression gradually decreased to P14.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Weak		3F
TS28: hippocampus	Moderate		3F	Expression gradually decreased to P14.
TS28: thalamus	Weak		3F	Expression decreased throughout thalamus.
TS28: pontine nucleus	Weak		3F	Expression gradually decreased to P14.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Weak	Regionally restricted	3G	Expression in layers II and II of future somatosensory area and future visual area of neocortex.
TS28: subiculum	Weak		3G
TS28: thalamic nucleus	Weak	Regionally restricted	3G	Expression in persisted in centrolateral thalamic nuclei on P10.
TS28: inferior colliculus	Weak		3G	Expression gradually decreased to P14.
TS28: pontine nucleus	Weak		3G	Expression gradually decreased to P14.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Weak
TS28: cerebellum	Absent

Structure	Level	Pattern	Image	Note
TS28: neocortex	Absent		3H
TS28: hippocampus	Absent		3H	Expression in hippocampus disappeared by P14.
TS28: inferior colliculus	Absent		3H	Overall expression disappeared by P14.
TS28: pontine nucleus	Absent		3H	Overall expression disappeared by P14.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent
TS28: thalamus	Absent

Structure	Level	Pattern	Image	Note
TS27: neocortex	Very strong	Widespread	4A	Prominent widespread expression in neocortex.
TS27: hippocampus	Strong		4A	Prominent expression in hippocampus.
TS27: thalamus	Very strong		4A	Prominent expression in thalamus.

Structure	Level	Pattern	Image	Note
TS27: piriform cortex	Moderate		4C
TS27: cerebellum	Moderate	Regionally restricted	4C	High levels of expression just below molecular layer.
TS27: deep cerebellar nucleus	Strong		4C
TS27: cerebellum internal granule cell layer	Weak		4C
TS27: cerebellum white matter	Weak		4C
TS27: cerebellum external granule cell layer	Absent		4C

Structure	Level	Pattern	Image	Note
TS27: cortical layer IV	Very strong		4D
TS27: cortical plate	Very strong		4D
TS27: cortical layer V	Moderate		4D
TS27: neocortex layer	Present	Regionally restricted	4D	Authors report low levels of expression in remaining layers of neocortex.

Structure	Level	Pattern	Image	Note
TS27: hippocampus region	Strong	Regionally restricted	4E	Prominent expression in hippocampus. Authors report ranking of signal intensity as CA3 < CA2 < CA1.
TS27: dentate gyrus	Weak		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS27: retina layer	retinal ganglion cell	Present	Regionally restricted	4F	Expression markedly enrighed in retinal ganglion cells in inner layer of retina.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Moderate		5A

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Moderate		5C

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak		5E	Expression in cerebellum decreases after P3.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		5G	Expression in cerebellum disappeared by P7.

Structure	Level	Pattern	Image	Note
TS27: pontine nucleus	Strong		6A
TS27: neocortex	Strong		6A
TS27: corpus striatum	Moderate		6A
TS27: piriform cortex	Moderate		6A