Ddit3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:250104 Flodby P, et al., The 78-kD Glucose-Regulated Protein Regulates Endoplasmic Reticulum Homeostasis and Distal Epithelial Cell Survival during Lung Development. Am J Respir Cell Mol Biol. 2016 Jul;55(1):135-49
Assay type:	Western blot
MGI Accession ID:	MGI:7704209
Gene symbol:	Ddit3
Gene name:	DNA-damage inducible transcript 3

Antibody:	DDIT3/GADD153/CHOP Antibody (B-3): sc-7351
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 4D CHOP

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	30 kDa (b)	Genetic Background	Mutant Allele(s)	Sex	Note
f/f	E18.0 (a)	TS26: lung	Weak	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee	Not Specified
f/f	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee	Not Specified
f/f	E18.0 (a)	TS26: lung	Weak	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee	Not Specified
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Weak	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.
cKO	E18.0 (a)	TS26: lung	Present	involves: 129 * C57BL/6 * DBA/2	Hspa5^tm1Alee/Hspa5^tm1Alee, Tg(Sftpc-cre)1Blh/0	Not Specified	Conditional mutant.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) An approximately 2.5-fold increase in expression in the mutant mice compared with controls (quantified in Fig 4E).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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