Gene Expression Data
Assay Details
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Assay
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Reference:
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J:259558
Bhattacharyya S, et al., Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development. Sci Rep. 2016 Feb 17;6:20727
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7703801
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Antibody:
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74.5A5
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
5 WT:
embryonic day 9.5
(more  )
Note: at the branchial level
Specimen
5 WT:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the branchial level
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Specimen
5 -/-:
(close )
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Genetic Background:
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B6.Cg-Ehd1tm1.2Haba
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Mutant Allele(s):
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Ehd1tm1.2Haba/Ehd1tm1.2Haba
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the branchial level
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
5_-/- Nkx2.2
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Increased expression in the ventral region, compared with the wild-type.
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Specimen
S6A WT:
embryonic day 9.5
(more )
Note: at the cranial level
Specimen
S6A WT:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the cranial level
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Specimen
S6A -/-:
(close )
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Genetic Background:
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B6.Cg-Ehd1tm1.2Haba
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Mutant Allele(s):
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Ehd1tm1.2Haba/Ehd1tm1.2Haba
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the cranial level
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
S6A -/- Nkx2.2
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Increased expression in the ventral region, compared with the wild-type.
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Specimen
S6B WT:
embryonic day 9.5
(more )
Note: at the lumbar level
Specimen
S6B WT:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the lumbar level
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Specimen
S6B -/-:
(close )
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Genetic Background:
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B6.Cg-Ehd1tm1.2Haba
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Mutant Allele(s):
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Ehd1tm1.2Haba/Ehd1tm1.2Haba
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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at the lumbar level
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
S6B -/- Nkx2.2
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Increased expression in the ventral region, compared with the wild-type.
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Analysis Tools