Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45357
Jemtland R, et al., Heterogeneity among cells that express osteoclast-associated genes in developing bone. Endocrinology. 1998 Jan;139(1):340-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7703512
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Gene symbol:
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Csf1
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Gene name:
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colony stimulating factor 1 (macrophage)
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Probe:
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M-CSF probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1g:
embryonic day 15.0
(more  )
Specimen
1g:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: epiphyseal plate hypertrophic zone |
Present |
Regionally restricted |
1g
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Expression in zone of hypertrophic chondrocytes in cartilage core.
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| TS23: tibia |
Present |
Regionally restricted |
1g
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Expression in zone of hypertrophic chondrocytes in cartilage core.
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Specimen
2m/n:
embryonic day 16.0
(more )
Specimen
2m/n:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
2m
,
2n
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Expression is widely distributed, most intense where future marrow cavity will form.
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| TS24: epiphyseal plate hypertrophic zone |
Present |
Regionally restricted |
2n
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2m
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Expression extends into lower part of hypertrophic chondrocyte zone.
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Specimen
3f:
embryonic day 17.0
(more )
Specimen
3f:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
3f
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Expression in bone marrow and along bone surfaces,.
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| TS25: epiphyseal plate ossification zone |
Present |
Regionally restricted |
3f
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Expression partly overlapping zone of hypertrophic chondrocytes.
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Analysis Tools