Gene Expression Data
Assay Details
Assay
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Reference:
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J:45357
Jemtland R, et al., Heterogeneity among cells that express osteoclast-associated genes in developing bone. Endocrinology. 1998 Jan;139(1):340-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7703507
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Gene symbol:
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Acp5
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Gene name:
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acid phosphatase 5, tartrate resistant
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Probe:
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TRAP probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1c:
embryonic day 15.0
(more )
Specimen
1c:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: tibia |
Present |
|
1c
|
|
Specimen
2e/f,g/h:
embryonic day 16.0
(more )
Specimen
2e/f,g/h:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: tibia |
Present |
Regionally restricted |
2f
,
2g
,
2e
,
2h
|
Expression restricted to cells along the inner layers of bone collar.
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Specimen
3c:
embryonic day 17.0
(more )
Specimen
3c:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: tibia |
Present |
Regionally restricted |
3c
|
Expression at scattered locations in marrow cavity.
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Specimen
4h/i:
embryonic day 17.0
(more )
Specimen
4h/i:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6h/i:
postnatal day 5
(more )
Specimen
6h/i:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: tibia |
Present |
Uniform |
6i
,
6h
|
Expression uniformly distributed in ossification center,
|
Specimen
7Ab/e:
postnatal day 15
(more )
Specimen
7Ab/e:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: tibia |
Present |
Uniform |
7Ab
,
7Ae
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Intensity of expression in uniform throughout bone.
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Specimen
7Bb/c:
postnatal day 15
(more )
Specimen
7Bb/c:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: tibia |
Present |
Widespread |
7Bb
,
7Bc
|
Expression co-localizes with TRAP throughout bone.
|
Specimen
7Be/f:
postnatal day 15
(more )
Specimen
7Be/f:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: tibia |
Present |
Regionally restricted |
7Be
,
7Bf
|
Some cells at chondro-osseous border continue to uniquely express type IV collagenase.
|
|