Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:45357
Jemtland R, et al., Heterogeneity among cells that express osteoclast-associated genes in developing bone. Endocrinology. 1998 Jan;139(1):340-9
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7703506
|
|
Gene symbol:
|
Csf1r
|
|
Gene name:
|
colony stimulating factor 1 receptor
|
|
Probe:
|
c-fma probe
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
1b:
embryonic day 15.0
(more  )
Specimen
1b:
(close  )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
Regionally restricted |
1b
|
Scattered expression in tissue surrounding cartilage core comprised of hypertrophic chondrocytes
|
| TS23: perichondrium |
Absent |
|
1b
|
No expression in perichondrium/periosteum.
|
| TS23: periosteum |
Absent |
|
1b
|
No expression in perichondrium/periosteum.
|
Specimen
2a/b,c/d:
embryonic day 16.0
(more )
Specimen
2a/b,c/d:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
2d
,
2a
,
2b
,
2c
|
Expression in cells along the inner layers of bone collar. Expression also in soft tissue.
|
Specimen
3b:
embryonic day 17.0
(more )
Specimen
3b:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
3b
|
Expression at scattered locations in marrow cavity.
|
Specimen
4b/c:
embryonic day 17.0
(more )
Specimen
4b/c:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
4c
,
4b
|
Colocalization with TRAP expresion inside skeleton.
|
Specimen
5e/f:
embryonic day 18.0
(more )
Specimen
5e/f:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
5e
,
5f
|
Expression in primary spongiosa.
|
Specimen
6b/c:
postnatal day 5
(more )
Specimen
6b/c:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
|
6b
,
6c
|
|
Specimen
7Aa/d:
postnatal day 15
(more )
Specimen
7Aa/d:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
postnatal day 15
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Uniform |
7Ad
,
7Aa
|
Intensity of expression in uniform throughout bone.
|
|
Analysis Tools