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Gene Expression Data
Assay Details
Assay
Reference: J:63337 Lu H, et al., Alteration in the expression of bone morphogenetic protein-2,3,4,5 mRNA during pathogenesis of cleft palate in BALB/c mice. Arch Oral Biol. 2000 Feb;45(2):133-40
Assay type: RNA in situ
MGI Accession ID: MGI:7665351
Gene symbol: Bmp4
Gene name: bone morphogenetic protein 4
Probe: pGEX-BMP-4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4: embryonic day 13.5 (more )
Note: Summarized in Table 1.
Structure Level Pattern Image Note
TS21: palatal shelf epithelium Strong 4
TS21: palatal shelf mesenchyme Strong Regionally restricted 4 Expressed in the dispersed mesenchymal cells. Highly condensing cells showed scattered, weak positive staining.
Specimen 6: embryonic day 13.5 (more )
Note: Summarized in Table 1.
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Strong Regionally restricted 6 Expressed in the dispersed mesenchymal cells. Highly condensing cells showed scattered, weak positive staining.
Specimen 7: embryonic day 14.5 (more )
Note: Summarized in Table 1.
Structure Cell Type Level Pattern Image Note
TS22: palatal shelf mesenchyme Moderate 7 Expressed in the dispersed and highly condensing cells.
TS22: palatal shelf mesenchyme osteoblast Present 7 Expressed in cells undergoing differentiation into osteoblasts.
Specimen 8: embryonic day 15.5 (more )
Note: Summarized in Table 1.
Structure Level Pattern Image Note
TS23: palatal shelf epithelium Absent 8
TS23: palatal shelf mesenchyme Strong Regionally restricted 8 Expressed in the highly condensing cells. Staining in dispersed mesenchymal cells was weak. The number of positive cells in mesenchyme decreased slightly compared with E13.5.
Specimen 9: embryonic day 15.5 (more )
Note: Summarized in Table 1.
Structure Cell Type Level Pattern Image Note
TS23: palatal shelf mesenchyme Strong Regionally restricted 9 Expressed in the highly condensing cells. Staining in dispersed mesenchymal cells was weak.
TS23: palatal shelf mesenchyme osteoblast Strong Regionally restricted 9 Expression in differentiated osteoblasts along the newly formed trabecular matrix increased.
Specimen not shown: embryonic day 14.5 (more )
Note: Summarized in Table 1.
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Absent
Specimen not shown: embryonic day 16.5 (more )
Note: Summarized in Table 1.
Structure Cell Type Level Pattern Image Note
TS24: palate epithelium Absent
TS24: palate mesenchyme Strong Regionally restricted Expressed in the highly condensing cells. Staining in dispersed mesenchymal cells was scattered and weak.
TS24: palate mesenchyme osteoblast Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
MGI 6.24 		The Jackson Laboratory