Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7665302
|
|
Gene symbol:
|
Nrp1
|
|
Gene name:
|
neuropilin 1
|
|
Probe:
|
Nrp1_riboprobe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The authors analyzed data from the Allen Developing Mouse Brain.
|
|
|
Results
|
Specimen
11a:
embryonic day 18.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11a:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11a
|
Expression was detected in intermediate and superficial strata of the preisthmus.
|
| TS26: periaqueductal grey |
Present |
Regionally restricted |
11a
|
The portion of the periaqueductal gray near the preisthmus has less signal, but the portion under the superior colliculus seemed specifically labeled.
|
| TS26: inferior colliculus |
Present |
Regionally restricted |
11a
|
Expression was detected in the inferior colliculus shell.
|
Specimen
11b:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11b:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11b
|
Expression was detected in intermediate and superficial strata of the preisthmus.
|
| TS26: periaqueductal grey |
Present |
Regionally restricted |
11b
|
The portion of the periaqueductal gray near the preisthmus has less signal, but the portion under the superior colliculus seemed specifically labeled.
|
| TS26: inferior colliculus |
Present |
Regionally restricted |
11b
|
Expression was detected in the inferior colliculus shell.
|
Specimen
11c:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11c:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11c
|
Expression was detected in intermediate and superficial strata of the preisthmus.
|
| TS26: periaqueductal grey |
Present |
Regionally restricted |
11c
|
The portion of the periaqueductal gray near the preisthmus has less signal, but the portion under the superior colliculus seemed specifically labeled.
|
| TS26: inferior colliculus |
Present |
Regionally restricted |
11c
|
Expression was detected in the inferior colliculus shell.
|
Specimen
11d:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11d:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11d
|
Expression was detected in intermediate and superficial strata of the preisthmus.
|
| TS26: periaqueductal grey |
Present |
Regionally restricted |
11d
|
The portion of the periaqueductal gray near the preisthmus has less signal, but the portion under the superior colliculus seemed specifically labeled.
|
| TS26: inferior colliculus |
Present |
Regionally restricted |
11d
|
Expression was detected in the inferior colliculus shell.
|
|
Analysis Tools