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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664607
Gene symbol: Ebf3
Gene name: early B cell factor 3
Probe: Ebf3_riboprobe1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 7a: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: superior colliculus Present 7a
TS23: mesomere 2 Present 7a
TS23: inferior colliculus Present Single cells 7a The inferior colliculus/brachium of the inferior colliculus had signal in a few dispersed cells, but was otherwise negative.
TS23: midbrain-hindbrain junction Present Regionally restricted 7a There was a negative boundary with the isthmus where a patch of signal appears in the mantle.
Specimen 7b: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: mesomere 2 Present 7b
TS23: superior colliculus Present 7b
TS23: midbrain Present Regionally restricted 7b Extensive labeling of the periaqueductal gray formation was observed.
TS23: inferior colliculus Present Single cells 7b The inferior colliculus had signal in a few dispersed cells, but was otherwise negative.
Specimen 9j: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: superior colliculus Present 9j
TS26: mesomere 2 Present 9j
TS26: midbrain-hindbrain junction Present Regionally restricted 9j Expression was detected in isolated isthmic cell masses.
Specimen 13n: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: midbrain Present Regionally restricted 13n Selective labeling of the subbrachial nucleus was observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory