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Gene Expression Data
Assay Details
Assay
Reference: J:345230 Garis M, et al., Expression of Nephronectin in the Descemet's membrane of mouse corneas during development and adult homeostasis. Exp Eye Res. 2024 Jan 19;240:109797
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7664565
Gene symbol: Npnt
Gene name: nephronectin
Antibody: anti-Npnt (AF4298)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1D: embryonic day 14.5 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS22: basement membrane Present Regionally restricted 1D Expression is in the basement membrane of the corneal epithelium.
TS22: cornea epithelium Present Regionally restricted 1D Expression is in the basement membrane of the corneal epithelium.
TS22: cornea Present Regionally restricted 1D Expression is in the region of the presumptive corneal endothelium.
TS22: retina Present Regionally restricted 1D Expression is adjacent to the retinal pigment epithelium.
Specimen 1E: embryonic day 18.5 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS26: eyelid mesenchyme Present 1E
TS26: basement membrane Present Regionally restricted 1E Expression is in the basement membrane of the corneal epithelium and endothelium.
TS26: cornea endothelium Present Regionally restricted 1E Expression is in the basement membrane of the corneal epithelium and endothelium.
TS26: cornea epithelium Present Regionally restricted 1E Expression is in the basement membrane of the corneal epithelium and endothelium.
Specimen 1F: postnatal adult (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS28: Descemet's membrane Strong 1F
TS28: basement membrane Present Regionally restricted 1F Expression is in the basement membrane of the corneal endothelium.
TS28: cornea endothelium Present Regionally restricted 1F Expression is in the basement membrane of the corneal endothelium.
Specimen 2C: embryonic day 18.5 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS26: Descemet's membrane Present 2C
Specimen 3C: postnatal day 0 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS27: Descemet's membrane Present 3C
Specimen 3H: postnatal day 4 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 3H
Specimen 4D: postnatal day 8 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 4D
Specimen 4I: postnatal day 10 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 4I
Specimen 4N: postnatal day 14 (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 4N
Specimen 5A: postnatal adult (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid). Double labeled: green - Npnt; red - Lama1 (assay MGI:7664570).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 5A , 5C
Specimen 5E: postnatal adult (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid). Double labeled: green - Npnt; red - Col8a2 (assay MGI:7664571).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 5G , 5E
Specimen 5I: postnatal adult (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid). Double labeled: green - Npnt; red - Hspg2 (assay MGI:7664572).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 5K , 5I
Specimen 5M: postnatal adult (more )
Note: Fixative was methanol-acetic acid (97% Methanol/3% acetic acid). Double labeled: green - Npnt; red - Col4a1 (assay MGI:7664573).
Structure Level Pattern Image Note
TS28: Descemet's membrane Present 5M , 5O
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory