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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7664537
Gene symbol: Tcf7l2
Gene name: transcription factor 7 like 2, T cell specific, HMG box
Probe: Tcf7l2_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 3v: embryonic day 11.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS19: midbrain Weak 3v
TS19: midbrain-hindbrain junction Weak 3v
Specimen 3w: embryonic day 12.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present Regionally restricted 3w Positive mantle cells started to aggregate subpially, particularly in an intermediate domain of the midbrain lying rostral to the sulcal area.
Specimen 5aa: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5aa Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5aa Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5aa Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5aa
TS22: midbrain-hindbrain junction Absent 5aa
Specimen 5bb: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5bb Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5bb Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5bb Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5bb
TS22: midbrain-hindbrain junction Absent 5bb
Specimen 5cc: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5cc Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5cc Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5cc Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5cc
TS22: midbrain-hindbrain junction Absent 5cc
Specimen 5dd: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5dd Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5dd Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5dd Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5dd
TS22: midbrain-hindbrain junction Absent 5dd
Specimen 5ee: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5ee Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5ee Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5ee Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5ee
TS22: midbrain-hindbrain junction Absent 5ee
Specimen 5ff: embryonic day 13.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5ff Expression was detected in the superior colliculus and inferior colliculus mantle primordia, leaving negative the preisthmus and the caudal sulcal area.
TS22: inferior colliculus Present Regionally restricted 5ff Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: superior colliculus Present Regionally restricted 5ff Expression was detected in the superior colliculus and inferior colliculus mantle primordia.
TS22: mesomere 2 Absent 5ff
TS22: midbrain-hindbrain junction Absent 5ff
Specimen 8i: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8i Labeling occupies the whole mantle.
TS23: superior colliculus Present 8i
TS23: mesomere 2 Present Single cells 8i The preisthmus was essentially negative except for a few periventricular cells.
Specimen 8j: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8j Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus Present 8j
TS23: mesomere 2 Present Single cells 8j The preisthmus was essentially negative except for a few periventricular cells.
Specimen 8k: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8k Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus Present 8k
TS23: mesomere 2 Present Single cells 8k The preisthmus was essentially negative except for a few periventricular cells.
Specimen 8l: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: inferior colliculus Present Regionally restricted 8l Labeling occupies the whole mantle, while more rostrally, possibly at levels through the brachium of the inferior colliculus nucleus, it appears separated by a negative periventricular stratum from the corresponding positive ventricular zone.
TS23: superior colliculus Present 8l
TS23: mesomere 2 Present Single cells 8l The preisthmus was essentially negative except for a few periventricular cells.
Specimen 11m: embryonic day 16.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS24: inferior colliculus Present 11m
TS24: midbrain Present Regionally restricted 11m Continuous expression was detected from the core of the inferior colliculus into the immature dorsomedial toral area, leaving the roof plate negative.
Specimen 11n: embryonic day 16.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS24: inferior colliculus Present 11n
TS24: midbrain Present Regionally restricted 11n Continuous expression was detected from the core of the inferior colliculus into the immature dorsomedial toral area, leaving the roof plate negative.
Specimen 11o: embryonic day 16.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS24: inferior colliculus Present 11o
TS24: midbrain Present Regionally restricted 11o The preisthmus appears negative, but a remnant of the positive toral area was present.
TS24: mesomere 2 Absent 11o
Specimen 11p: embryonic day 16.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS24: inferior colliculus Present 11p
TS24: midbrain Present Regionally restricted 11p The preisthmus appears negative, but a remnant of the positive toral area was present.
TS24: mesomere 2 Absent 11p
Specimen 11q: embryonic day 17.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: inferior colliculus Present 11q
TS25: midbrain Present Regionally restricted 11q Expression pattern was similar to E16.5.
Specimen 11r: embryonic day 17.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: inferior colliculus Present 11r
TS25: midbrain Present Regionally restricted 11r Expression pattern was similar to E16.5.
Specimen 11s: embryonic day 17.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: inferior colliculus Present 11s
TS25: midbrain Present Regionally restricted 11s Expression pattern was similar to E16.5.
Specimen 11v: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: inferior colliculus Present 11v
TS26: midbrain Present Regionally restricted 11v Expression pattern was similar to E16.5.
Specimen 11w: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: inferior colliculus Present 11w
TS26: midbrain Present Regionally restricted 11w Expression pattern was similar to E16.5.
Specimen 11x: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: inferior colliculus Present 11x
TS26: midbrain Present Regionally restricted 11x Expression pattern was similar to E16.5.
Specimen 11y: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: inferior colliculus Present 11y
TS26: midbrain Present Regionally restricted 11y Expression pattern was similar to E16.5.
Specimen 13d: postnatal day 1 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS27: inferior colliculus Present 13d
TS27: midbrain Present Regionally restricted 13d Expression was detected in the toral region.
Specimen 13e: postnatal day 2 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS27: inferior colliculus Present 13e
TS27: midbrain Present Regionally restricted 13e Expression was detected in the toral region.
Specimen 13f: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: inferior colliculus Present 13f
TS28: midbrain Present Regionally restricted 13f Expression was detected in the toral region.
Specimen 13j: postnatal day 4 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: midbrain Present Regionally restricted 13j Expression was detected in the former toral bulges, which show marked flattening.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory