Gene Expression Data
Assay Details
Assay
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Reference:
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J:93103
Diaz D, et al., Effect of methylmercury on glutamate-cysteine ligase expression in the placenta and yolk sac during mouse development. Reprod Toxicol. 2004 Nov;19(1):117-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664465
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Gene symbol:
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Gclc
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Gene name:
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glutamate-cysteine ligase, catalytic subunit
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Probe:
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Gclc cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A gd10 embryo:
embryonic day 10.5
(more )
Specimen
3A gd10 embryo:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: spongiotrophoblast |
Present |
|
3A Gclc gd10
|
Expression was roughly constant from gd 10 to gd16.
|
Specimen
5 gd10 maternal:
postnatal week 5-6
(more )
Specimen
5 gd10 maternal:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5-6
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
|
3A Gclc gd10
|
|
Specimen
3A gd12 embryo:
embryonic day 12.5
(more )
Specimen
3A gd12 embryo:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spongiotrophoblast |
Present |
|
3A Gclc gd12
|
Expression was roughly constant from gd 10 to gd16.
|
Specimen
5 gd12 maternal:
postnatal week 5-6
(more )
Specimen
5 gd12 maternal:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5-6
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
|
3A Gclc gd12
|
|
Specimen
3A gd14 embryo:
embryonic day 14.5
(more )
Specimen
3A gd14 embryo:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spongiotrophoblast |
Present |
|
3A Gclc gd14
|
Expression was roughly constant from gd 10 to gd16.
|
Specimen
5 gd14 maternal:
postnatal week 5-6
(more )
Specimen
5 gd14 maternal:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5-6
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
|
3A Gclc gd14
|
|
Specimen
3A gd16 embryo:
embryonic day 16.5
(more )
Specimen
3A gd16 embryo:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spongiotrophoblast |
Present |
|
3A Gclc gd16
|
Expression was roughly constant from gd 10 to gd16.
|
Specimen
5 gd16 maternal:
postnatal week 5-6
(more )
Specimen
5 gd16 maternal:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5-6
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
|
3A Gclc gd16
|
|
Specimen
3A gd18 embryo:
embryonic day 18.5
(more )
Specimen
3A gd18 embryo:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5 gd18 maternal:
postnatal week 5-6
(more )
Specimen
5 gd18 maternal:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5-6
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
|
3A Gclc gd18
|
|
Specimen
3B:
embryonic day 18.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|