Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7664446
|
|
Gene symbol:
|
Trh
|
|
Gene name:
|
thyrotropin releasing hormone
|
|
Probe:
|
Trh_riboprobe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The authors analyzed data from the Allen Developing Mouse Brain.
|
|
|
Results
|
Specimen
3r:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3r:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
3r
|
Signal appeared selectively in a semilunar postmitotic mantle area that might correspond to the caudal sulcal area.
|
Specimen
11z:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11z:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11z
|
A singular basal preisthmic dot-like area of expression was seen periventricularly.
|
Specimen
11aa:
embryonic day 18.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
11aa:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mesomere 2 |
Present |
Regionally restricted |
11aa
|
A Trh-positive distinct cell group in the ventral periaqueductal gray associated with the preisthmus, out of which less numerous similar cells spread out laterally and ventrally.
|
| TS26: midbrain |
Present |
Regionally restricted |
11aa
|
A Trh-positive distinct cell group in the ventral periaqueductal gray associated with the preisthmus, out of which less numerous similar cells spread out laterally and ventrally.
|
| TS26: periaqueductal grey |
Present |
Regionally restricted |
11aa
|
A Trh-positive distinct cell group in the ventral periaqueductal gray associated with the preisthmus, out of which less numerous similar cells spread out laterally and ventrally.
|
|
Analysis Tools