Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7664435
|
|
Gene symbol:
|
Nkx2-2
|
|
Gene name:
|
NK2 homeobox 2
|
|
Probe:
|
Nkx2-2_riboprobe3
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The authors analyzed data from the Allen Developing Mouse Brain.
|
|
|
Results
|
Specimen
2bb:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
2bb:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain |
Present |
Regionally restricted |
2bb
|
The longitudinal route of expression ended at the caudal midbrain, where it was deflected dorsalward just in front of the caudal sulcal domain.
|
| TS19: mesomere 1 |
Present |
|
2bb
|
|
Specimen
6o:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6o:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
Present |
Patchy |
6o
|
Positive cell patches were detected in the dorsal inferior colliculus mass.
|
Specimen
6p:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6p:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
Present |
Patchy |
6p
|
Positive cell patches were detected in the dorsal inferior colliculus mass.
|
Specimen
6q:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6q:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inferior colliculus |
Present |
Patchy |
6q
|
Positive cell patches were detected in the dorsal inferior colliculus mass.
|
Specimen
6r:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6r:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
6r
|
More ventrally there is more compact signal associated with the caudal sulcal area along the dorsoventral dimension of the preisthmus.
|
| TS22: midbrain |
Present |
Regionally restricted |
6r
|
More ventrally there is more compact signal associated with the caudal sulcal area along the dorsoventral dimension of the preisthmus.
|
Specimen
6s:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6s:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
6s
|
More ventrally there is more compact signal associated with the caudal sulcal area along the dorsoventral dimension of the preisthmus.
|
| TS22: midbrain |
Present |
Regionally restricted |
6s
|
More ventrally there is more compact signal associated with the caudal sulcal area along the dorsoventral dimension of the preisthmus.
|
Specimen
6t:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6t:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
|
Present |
Regionally restricted |
6t
|
Signal showed the confluence of the preisthmic transverse pattern with the longitudinal band coursing along the alar-basal boundary.
|
| TS22: midbrain |
neuron |
Present |
Regionally restricted |
6t
|
A clump of periaqueductal positive neurons migrated dorsalward.
|
| TS22: midbrain tegmentum |
|
Present |
Regionally restricted |
6t
|
Radial ventral distribution of Nkx2.2-positive cells in the laterorubral tegmental area was observed.
|
Specimen
6u:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6u:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
|
Present |
Regionally restricted |
6u
|
Signal showed the confluence of the preisthmic transverse pattern with the longitudinal band coursing along the alar-basal boundary.
|
| TS22: midbrain |
neuron |
Present |
Regionally restricted |
6u
|
A clump of periaqueductal positive neurons migrated dorsalward.
|
| TS22: midbrain tegmentum |
|
Present |
Regionally restricted |
6u
|
Radial ventral distribution of Nkx2.2-positive cells in the laterorubral tegmental area was observed.
|
|
Analysis Tools