Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50415
Denis-Donini S, et al., Localization of calcitonin gene-related peptide mRNA in developing olfactory axons. Cell Tissue Res. 1998 Oct;294(1):81-91
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664422
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Probe:
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psp64
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Horseradish peroxidase
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Results
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Specimen
1b:
embryonic day 14.0
(more  )
Note: dorsal olfactory axon bundle
Specimen
1b:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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dorsal olfactory axon bundle
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Specimen
1c:
embryonic day 14.0
(more )
Note: high magnification
Specimen
1c:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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high magnification
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Specimen
2b:
embryonic day 13.0
(more )
Specimen
2b:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Specimen
2c:
embryonic day 13.0
(more )
Specimen
2c:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: olfactory nerve |
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Present |
Punctate |
2c
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The electron-dense precipitates had the same round shape whatever the plane of the section, longitudinal or transversal, indicating that they are defined discrete spots, not filamentous in structure.
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| TS21: olfactory nerve |
olfactory ensheathing cell |
Absent |
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2c
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Specimen
2d:
embryonic day 13.0
(more )
Specimen
2d:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory nerve |
Present |
Regionally restricted |
2d
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Only a few in the most proximal axonal segment exiting the lamina propria.
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Specimen
3a:
embryonic day 13.0
(more )
Note: high magnification
Specimen
3a:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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high magnification
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory nerve |
Present |
Punctate |
3a
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The precipitates look like aggregates of different density, conferring on them a granular aspect.
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Specimen
3b:
embryonic day 13.0
(more )
Note: high magnification
Specimen
3b:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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high magnification
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory nerve |
Present |
Punctate |
3b
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In transverse sections, the clusters usually appeared arranged in small groups, often located in a slightly eccentric position with a pattern comparable to that of intermediate filaments.
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Specimen
not shown:
embryonic day 15.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: olfactory nerve |
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Present |
Punctate |
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Expressed in developing olfactory axons but not in the ensheathing cells. Labelled axons
were not present in all bundles but are predominantly located in the most dorsal ones. The probability of envisioning precipitates increased as the more distal segments of the axon were reached.
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| TS23: olfactory nerve |
olfactory ensheathing cell |
Absent |
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