Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47739
Nohr D, et al., Distribution of the CD15 epitope in the mammalian developing lung is opposite in mouse compared with human. Differentiation. 1998 May;63(1):43-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7664110
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Gene symbol:
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Fut4
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Gene name:
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fucosyltransferase 4
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Antibody:
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HB-78 (MMA)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
not shown:
embryonic day 9.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trachea |
Present |
Regionally restricted |
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Only some tracheal cells were immunoreactive.
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Specimen
1A:
embryonic day 10.5
(more )
Specimen
1A:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trachea epithelium |
Strong |
Non-Uniform |
1A
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Intense immunoreactivity was detected, which was most prominent on the apical cell surfaces but was also found on the lateral cell membranes.
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| TS17: bronchus |
Weak |
Single cells |
1A
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Very few cells in the bronchial bud region exhibited a weak, spotty immunoreactivity on their apical surface.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trachea epithelium |
Present |
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| TS19: bronchus epithelium |
Present |
Regionally restricted |
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The proximal parts of the developing bronchi exhibited increasing immunoreactivity. More distal parts, representing proliferating regions of the bronchi, were immunonegative.
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trachea epithelium |
Present |
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1B
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| TS20: bronchus epithelium |
Present |
Regionally restricted |
1B
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The proximal parts of the developing bronchi exhibited increasing immunoreactivity. More distal parts, representing proliferating regions of the bronchi, were immunonegative.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trachea epithelium |
Present |
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| TS21: bronchus epithelium |
Present |
Regionally restricted |
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The proximal parts of the developing bronchi exhibited increasing immunoreactivity. More distal parts, representing proliferating regions of the bronchi, were immunonegative.
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Specimen
1C:
embryonic day 17.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: bronchus epithelium |
epithelial cell of lung |
Strong |
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1C
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| TS25: lobar bronchus epithelium |
epithelial cell of lung |
Strong |
Regionally restricted |
1C
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All airways down to segmental bronchi were strongly immunoreactive, while the distal branching buds were negative. Cytoplasmic staining was more and more prominent in the typical columnar cells of the lining epithelium in the proximal and medial parts of the bronchi, while the cuboidal epithelial cells in the distal, proliferating areas of the bronchi and bronchioli remained immunonegative.
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Specimen
1D:
embryonic day 17.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: lobar bronchus epithelium |
epithelial cell of lung |
Strong |
Regionally restricted |
1D
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All airways down to segmental bronchi were strongly immunoreactive, while the distal branching buds were negative. Cytoplasmic staining was more and more prominent in the typical columnar cells of the lining epithelium in the proximal and medial parts of the bronchi, while the cuboidal epithelial cells in the distal, proliferating areas of the bronchi and bronchioli remained immunonegative.
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