Antibody:	Anti-TOB2 (N-20); sc-18551
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS21: lens fiber	Present	Regionally restricted	3a	In E13.5 lenses, distinct reactivity was detected in the differentiating primary and secondary fibers.
TS21: lens transition zone	Present	Regionally restricted	3a	Expression was detected in granular or vesicular staining in the transitional zone.
TS21: lens germinative zone	Weak	Regionally restricted	3a	Weak staining was detected in the germinative zone epithelium.
TS21: lens anterior epithelium	Ambiguous		3a	Little or no staining was observed in the anterior epithelium.

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Present	Regionally restricted	3c	Expression increased as the fiber cells underwent elongation. Immunoreactivity was predominantly present as cytoplasmic puncta, with larger and more intensely labelled granules evident in the cortical fibers.
TS22: lens transition zone	Present	Regionally restricted	3c	Expression was detected in granular or vesicular staining in the transitional zone.
TS22: lens germinative zone	Weak	Regionally restricted	3c	Weak staining was detected in the germinative zone epithelium.
TS22: lens anterior epithelium	Ambiguous		3c	Little or no staining was observed in the anterior epithelium.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present	Regionally restricted	3e	Expression increased as the fiber cells underwent elongation. Immunoreactivity was predominantly present as cytoplasmic puncta, with larger and more intensely labelled granules evident in the cortical fibers.
TS24: lens transition zone	Present	Regionally restricted	3e	Expression was detected in granular or vesicular staining in the transitional zone.
TS24: lens germinative zone	Weak	Regionally restricted	3e	Weak staining was detected in the germinative zone epithelium.
TS24: lens anterior epithelium	Ambiguous		3e	Little or no staining was observed in the anterior epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	lens fiber cell	Present	Regionally restricted	4b' , 4d' , 4d , 4b	Expression was most strongly localized to the anterior regions of the lens fibers cells. There was partial overlap with Eif3b in some of the cytoplasmic granules.