Antibody:	Anti-Dcp2 (ab28658)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Regionally restricted	2b , 2c	Expression was often observed in association with Tob1 throughout the vesicle, and in larger cytoplasmic complexes in posterior vesicle cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	lens fiber cell	Present	Regionally restricted	2f , 2e	Expression was detected in differentiating fiber cells in large complexes associated with Tob1.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: lens transition zone	lens fiber cell	Present	Regionally restricted	2j , 2h	In transitional zone fiber cells, expression was found in large cytoplasmic complexes with Tob1.
TS24: lens fiber	lens fiber cell	Present	Regionally restricted	2h , 2j	In more differentiated fiber cells of the lens cortex, immunoreactivity was associated with nuclei and co-localized with Tob1.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	lens fiber cell	Present	Regionally restricted	3b	Expression was detected in fiber cells in large cytoplasmic granules.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lens fiber	lens fiber cell	Present	Regionally restricted	3d	Expression was detected in fiber cells in large cytoplasmic granules.