Antibody:	Anti-TOB1 (E-1); sc-133095
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Punctate	2a , 2c	At E11.5, immunoreactivity was found throughout the lens vesicle as fine granular punctate staining, often in association with Dcp2. Larger cytoplasmic complexes of Tob1 and Dcp2 were observed in posterior vesicle cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	lens fiber cell	Present	Punctate	2f , 2d	Distinct large punctate immunoreactivity was detected in the elongating fiber cells. Co-localization with Dcp2 was observed.
TS21: lens anterior epithelium	anterior lens cell	Weak	Regionally restricted	2d , 2f	Weak granular immunoreactivity was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: lens germinative zone		Present	Regionally restricted	2j , 2g	At E16.5 distinct granular cytoplasmic staining was detected in the germinative zone cells.
TS24: lens transition zone	lens fiber cell	Present	Regionally restricted	2g , 2j	In transitional zone fiber cells, co-localization with DCP2 was found in large cytoplasmic complexes. Expression was also adjacent to DCP2 in large cytoplasmic complexes in fiber cells of the transitional zone.
TS24: lens fiber	lens fiber cell	Present	Punctate	2g , 2j	In more differentiated fiber cells of the lens cortex, co-localization was associated with nuclei.
TS24: lens anterior epithelium		Ambiguous		2g , 2j	Little or no staining was detected.