Cebpa Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:348284 Hassan D, et al., CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair. Nat Commun. 2024 May 16;15(1):4148
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7645863
Gene symbol:	Cebpa
Gene name:	CCAAT/enhancer binding protein alpha

Antibody:	Anti-C/EBPa (D56F10; 8178)
Detection system:	Secondary antibody

Results

Specimen 2A - E14.5: embryonic day 14.5 (more

)
Note: Double labeled: red - Cebpa; white - Cdh1 (assay MGI:7645862).

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Absent		2A E14.5 CEBPA/ECAD

Specimen 2A - E16.5: embryonic day 16.5 (more

)
Note: Double labeled: red - Cebpa; white - Cdh1 (assay MGI:7645862).

Structure	Level	Pattern	Image	Note
TS24: alveolus epithelium	Weak	Single cells	2A E16.5 CEBPA/ECAD	Weak, diffuse expression is seen in occasional cells.

Specimen 2A - E18.5: embryonic day 18.5 (more

)
Note: Double labeled: red - Cebpa; white - Cdh1 (assay MGI:7645862).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: alveolus epithelium	fetal pre-type II pulmonary alveolar epithelial cell	Present		2A E18.5 CEBPA/ECAD

Specimen S7E - E16.5 (left): embryonic day 16.5 (more

)
Note: Triple labeled: red - Cebpa; green - Hopx (assay MGI:7645865); magenta - Sox9 (assay MGI:7645864).

Structure	Level	Pattern	Image	Note
TS24: alveolus epithelium	Weak	Diffuse	S3E E16.5 HOPX/CEBPA , S3E E16.5 CEBPA , S3E E16.5 HOPX/CEBPA/SOX9	Weak, diffuse expression is seen in occasional cells.

Specimen S7E - E18.5 (left): embryonic day 18.5 (more

)
Note: Triple labeled: red - Cebpa; green - Hopx (assay MGI:7645865); magenta - Sox9 (assay MGI:7645864).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: alveolus epithelium	fetal pre-type II pulmonary alveolar epithelial cell	Present		S3E E18.5 CEBPA , S3E E18.5 HOPX/CEBPA , S3E E18.5 HOPX/CEBPA/SOX9

Specimen S7E - E16.5 (right): embryonic day 16.5 (more

)
Note: Double labeled: red - Cebpa; white - Cdh1 (assay MGI:7645862).

Structure	Level	Pattern	Image	Note
TS24: alveolus epithelium	Weak	Diffuse	S3E E16.5 CEBPA/ECAD	Weak, diffuse expression is seen in occasional cells.

Specimen S7E - E18.5 (right): embryonic day 18.5 (more

)
Note: Double labeled: red - Cebpa; white - Cdh1 (assay MGI:7645862).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: alveolus epithelium	fetal pre-type II pulmonary alveolar epithelial cell	Present		S3E E18.5 CEBPA/ECAD

Specimen S7F: embryonic day 18.5 (more

)
Note: Triple labeled: red - Cebpa; green - Hopx (assay MGI:7645865); blue - Lamp3 (assay MGI:7645866).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: alveolus epithelium	fetal pre-type II pulmonary alveolar epithelial cell	Present		S3F LAMP3/CEBPA/HOPX , S3F CEBPA/HOPX , S3F CEBPA/LAMP3

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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