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Gene Expression Data
Assay Details
Assay
Reference: J:153077 Satake H, et al., Spatio-temporal expression of activating transcription factor 5 in the skeletal development of mouse limb. Dev Growth Differ. 2009 Sep;51(7):669-76
Assay type: RNA in situ
MGI Accession ID: MGI:7645157
Gene symbol: Atf5
Gene name: activating transcription factor 5
Probe: Atf5 cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1a: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb pre-cartilage condensation Present Regionally restricted 1a Atf5
TS20: forelimb mesenchyme Present Regionally restricted 1a Atf5 Expression is in the mesenchymal cells contiguous to the cartilage primordia.

Specimen 1b: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: forelimb pre-cartilage condensation Present 1b Atf5
TS21: perichondrium Absent 1b Atf5

Specimen 2a: embryonic day 15.5 (more )
Structure Cell Type Level Pattern Image Note
TS23: forelimb cartilage condensation chondrocyte Present Regionally restricted 2a Expression is in proliferative and prehypertrophic chondrocytes within the developing cartilage elements.
TS23: radius osteoblast Present Regionally restricted 2a Expression is in osteoblasts populating the primary spongiosa.

Specimen 3: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: tibia osteoblast Present Regionally restricted 3a Expression is in osteoblasts lining the bone trabeculae.

Specimen 4A: postnatal day 14 (more )
Structure Cell Type Level Pattern Image Note
TS28: tibia epiphysis articular chondrocyte Present 4Ba , 4A Expression is in articular chondrocytes.
TS28: tibia epiphysis chondrocyte Present 4A , 4Bb Expression is in proliferative and prehypertrophic chondrocytes.
TS28: tibia epiphysis osteoblast Present 4Bc , 4A , 4Ba Expression is in osetoblasts.
TS28: tibia metaphysis osteoblast Present 4Bc , 4A , 4Ba Expression is in osetoblasts.

Specimen 5A: postnatal month 3 (more )
Structure Cell Type Level Pattern Image Note
TS28: tibia epiphysis chondrocyte Strong 5Ab , 5A Expression is in chondrocytes of epiphyseal cartilages.
TS28: tibia osteoblast Present Regionally restricted 5Ac , 5A Expression is in osteoblasts lining the bone trabeculae.
TS28: articular cartilage articular chondrocyte Weak 5A , 5Aa Expression is in articular chondrocytes.

Specimen Table 2 E12.5: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: forelimb pre-cartilage condensation chondrocyte Present Expression is in proliferative chondrocytes.

Specimen Table 2 E13.5: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: forelimb pre-cartilage condensation chondrocyte Present Expression is in proliferative chondrocytes.

Specimen Table 2 E14.5: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: forelimb pre-cartilage condensation chondrocyte Present Regionally restricted Expression is in proliferative chondrocytes.
TS22: forelimb pre-cartilage condensation hypertrophic chondrocyte Absent Expression is absent in hypertrophic chondrocytes.

Specimen Table 2 E15.5: embryonic day 15.5 (more )
Structure Cell Type Level Pattern Image Note
TS23: forelimb pre-cartilage condensation chondrocyte Present Expression is in proliferative chondrocytes.
TS23: forelimb bone osteoblast Present Expression is in osteoblasts.
TS23: forelimb pre-cartilage condensation hypertrophic chondrocyte Absent Expression is absent in hypertrophic chondrocytes.

Specimen Table 2 E16.5: embryonic day 16.5 (more )
Structure Cell Type Level Pattern Image Note
TS24: forelimb cartilage condensation chondrocyte Present Expression is in proliferative chondrocytes.
TS24: forelimb bone osteoblast Present Expression is in osteoblasts.
TS24: forelimb cartilage condensation hypertrophic chondrocyte Absent Expression is absent in hypertrophic chondrocytes.

Specimen Table 2 E18.5: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: forelimb cartilage condensation chondrocyte Present Expression is in proliferative chondrocytes.
TS26: forelimb bone osteoblast Present Expression is in osteoblasts.
TS26: forelimb cartilage condensation hypertrophic chondrocyte Absent Expression is absent in hypertrophic chondrocytes.

Specimen Table 2 PD1: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: forelimb chondrocyte Present Expression is in proliferative chondrocytes.
TS27: articular cartilage articular chondrocyte Present
TS27: forelimb bone osteoblast Present Expression is in osteoblasts.
TS27: forelimb hypertrophic chondrocyte Absent Absent in hypertrophic chondrocytes.

Specimen Table 2 PD7: postnatal day 7 (more )
Structure Cell Type Level Pattern Image Note
TS28: forelimb chondrocyte Present Expression is in proliferative chondrocytes.
TS28: articular cartilage articular chondrocyte Present
TS28: forelimb bone osteoblast Present Expression is in osteoblasts.
TS28: forelimb hypertrophic chondrocyte Absent Absent in hypertrophic chondrocytes.

Specimen Table 2 PD14: postnatal day 14 (more )
Structure Cell Type Level Pattern Image Note
TS28: forelimb chondrocyte Present Expression is in proliferative chondrocytes.
TS28: articular cartilage articular chondrocyte Present
TS28: forelimb bone osteoblast Present Expression is in osteoblasts.
TS28: forelimb hypertrophic chondrocyte Absent Absent in hypertrophic chondrocytes.

Specimen Table 2 PD28: postnatal day 28 (more )
Structure Cell Type Level Pattern Image Note
TS28: forelimb chondrocyte Present Expression is in proliferative chondrocytes.
TS28: articular cartilage articular chondrocyte Present
TS28: forelimb bone osteoblast Present Expression is in osteoblasts.
TS28: forelimb hypertrophic chondrocyte Absent Absent in hypertrophic chondrocytes.

Specimen Table 2 PM3: postnatal month 3 (more )
Structure Cell Type Level Pattern Image Note
TS28: forelimb chondrocyte Present Expression is in proliferative chondrocytes.
TS28: forelimb bone osteoblast Present Expression is in osteoblasts.
TS28: articular cartilage articular chondrocyte Weak
TS28: forelimb hypertrophic chondrocyte Absent Absent in hypertrophic chondrocytes.

Specimen not shown E14.5: embryonic day 14.5 (more )
Structure Cell Type Level Pattern Image Note
TS22: forelimb cartilage condensation chondrocyte Present Regionally restricted Expression is in proliferative and prehypertrophic chondrocytes within the developing cartilage elements.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory